Anti-BAX Antibody (Clone#OTI2A1)

Mouse monoclonal antibody

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筛选器： All WB IHC

M00183-4

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期： 5-7天

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Western blot analysis of anti-BAX antibody (M00183-4). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: human COLO-320 whole cell lysates,
Lane 4: human HT1080 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-BAX antigen affinity purified monoclonal antibody (M00183-4) at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for BAX at approximately 21 kDa. The expected band size for BAX is at 21 kDa.
all(3)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-BAX mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, M00183-4) (1:2000)
all(3)
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-BAX mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, M00183-4) (1:2000)
all(3)

产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-BAX Antibody (Clone#OTI2A1)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

Apoptosis regulator BAX; BAX

产品类型

Monoclonal

检验物种

human, mouse, rat

应用范围

WB, IHC

基因名称

BAX

克隆号

OTI2A1

宿主

Mouse

抗体亚型

IgG2b

免疫原

Human recombinant protein fragment corresponding to amino acids 1-172 of human BAX (NP_620116) produced in E.coli.

计算分子量

21 kDa

实际分子量

21 kDa

成分

PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

纯化方式

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

浓度

500 ug/ml

产品形态

Liquid

保存条件

Stable for 12 months from date of receipt. Store at -20°C as received.

背景资料

Apoptosis regulator BAX, also known as bcl-2-like protein 4, is a protein that in humans is encoded by the BAX gene. The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein forms a heterodimer with BCL2, and functions as an apoptotic activator. Additionally, this protein is reported to interact with, and increase the opening of, the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in membrane potential and the release of cytochrome c. The expression of this gene is regulated by the tumor suppressor P53 and has been shown to be involved in P53-mediated apoptosis. Multiple alternatively spliced transcript variants, which encode different isoforms, have been reported for this gene.

Uniprot ID

Q07812

文献引用格式

BAX Antibody (Clone#OTI2A1) (Boster Biological Technology, Wuhan, China. Catalog#M00183-4)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

BCL2-associated X protein

蛋白名全称

Apoptosis regulator BAX

实验方案>

实验步骤

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