Anti-VDR Antibody

筛选器： All WB FCM

• 

  Western blot analysis of VDR using anti-VDR antibody (A00210-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: SH-SY5Y whole cell lysates,
  Lane 2: U-937 whole cell lysates,
  Lane 3: Hacat whole cell lysates,
  Lane 4: HL-60 whole cell lysates,
  Lane 5: rat brain tissue lysates,
  Lane 6: PC-12 whole cell lysates,
  Lane 7: mouse brain tissue lysates,
  Lane 8: NIH/3T3 whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-VDR antigen affinity purified polyclonal antibody (A00210-1) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for VDR at approximately 60 kDa. The expected band size for VDR is at 48 kDa.

• 

  Flow Cytometry analysis of C6 cells using anti-VDR antibody (A00210-1).
  Overlay histogram showing C6 cells stained with A00210-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-VDR Antibody (A00210-1) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

• 

  Flow Cytometry analysis of U2OS cells using anti-VDR antibody (A00210-1).
  Overlay histogram showing U2OS cells stained with A00210-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-VDR Antibody (A00210-1) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

• 

  Western blot analysis of VDR using anti-VDR antibody (A00210-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: SH-SY5Y whole cell lysates,
  Lane 2: U-937 whole cell lysates,
  Lane 3: Hacat whole cell lysates,
  Lane 4: HL-60 whole cell lysates,
  Lane 5: rat brain tissue lysates,
  Lane 6: PC-12 whole cell lysates,
  Lane 7: mouse brain tissue lysates,
  Lane 8: NIH/3T3 whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-VDR antigen affinity purified polyclonal antibody (A00210-1) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for VDR at approximately 60 kDa. The expected band size for VDR is at 48 kDa.

• 

  Flow Cytometry analysis of C6 cells using anti-VDR antibody (A00210-1).
  Overlay histogram showing C6 cells stained with A00210-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-VDR Antibody (A00210-1) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

• 

  Flow Cytometry analysis of U2OS cells using anti-VDR antibody (A00210-1).
  Overlay histogram showing U2OS cells stained with A00210-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-VDR Antibody (A00210-1) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.