Anti-VDR Antibody (Clone#AAGE-22)

Rabbit monoclonal antibody

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筛选器： All WB IP

BM5055

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of anti-VDR antibody (BM5055). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: mouse kidney tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-VDR antigen affinity purified monoclonal antibody (BM5055) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for VDR at approximately 48 kDa. The expected band size for VDR is at 48 kDa.
all(3)
All lanes use the Antibody for 1 hour at room temperature.
all(3)
all(3)

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产品简介>

产品名称

Anti-VDR Antibody (Clone#AAGE-22)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

NR1I1; VDR; Vitamin D3 receptor

产品类型

Monoclonal

检验物种

human, mouse, rat

应用范围

WB, IP

基因名称

VDR

克隆号

AAGE-22

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthesized peptide derived from human Vitamin D Receptor

计算分子量

48 kDa

实际分子量

48 kDa

成分

500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.

纯化方式

Affinity-chromatography

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

VDR(Vitamin D Receptor), also known as Vitamin D Hormone Receptor, is a member of the nuclear receptor family of transcription factors. Labuda et al.(1991) assigned the VDR gene to 12q12-q14 by in situ hybridization. Using mutation analysis, Jurutka et al.(2000) characterized arg18/arg22, VDR residues immediately N-terminal of the first DNA-binding zinc finger, as vital for contact with the general transcription factor IIB(TFIIB). A natural polymorphic variant of VDR, termed F/M4(missing a FokI restriction site), which lacks only the first 3 amino acids(including glu2), interacted more efficiently with TFIIB and also possessed elevated transcriptional activity compared with the full-length(f/M1) receptor. Shah et al.(2006) stated that the signaling and oncogenic activity of beta-catenin(CTNNB1) can be repressed by activation of VDR. Conversely, high levels of beta-catenin can potentiate the transcriptional activity of 1,25-dihydroxyvitamin D3.

Uniprot ID

P11473

文献引用格式

VDR Antibody (Clone#AAGE-22) (Boster Biological Technology, Wuhan, China. Catalog#BM5055)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

Vitamin D3 receptor

实验方案>

实验步骤

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