Anti-TBK1 Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC ELISA

A00261-1

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of TBK1 using anti-TBK1 antibody (A00261-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: 293T whole cell lysates,
Lane 2: HEPG2 whole cell lysates,
Lane 3: LNCAP whole cell lysates,
Lane 4: rat brain tissue lysates,
Lane 5: C6 whole cell lysates,
Lane 6: mouse brain tissue lysates,
Lane 7: NEURO-2A whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-TBK1 antigen affinity purified polyclonal antibody (A00261-1) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for TBK1 at approximately 84 kDa. The expected band size for TBK1 is at 84 kDa.
all(3)
IHC analysis of TBK1 using anti-TBK1 antibody (A00261-1).
TBK1 was detected in a paraffin-embedded section of mouse testis tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-TBK1 Antibody (A00261-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(3)
all(3)

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产品简介>

产品名称

Anti-TBK1 Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

FLJ11330; NAK; NF kappa B activating kinase; T2K; TANK binding kinase 1; TBK1

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, ELISA

基因名称

TBK1

宿主

Rabbit

抗体亚型

IgG

免疫原

E.coli-derived human NAK/TBK1 recombinant protein (Position: M1-L729).

计算分子量

84 kDa

实际分子量

84 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

Serine/threonine-protein kinase TBK1, also called TANK-binding kinase 1 or NF-kappa-B-activating kinase is an enzyme that in humans is encoded by the TBK1 gene. The gene was assigned to human chromosome 12q14.2. Serine/threonine kinase plays an essential role in regulating inflammatory responses to foreign agents. TBK1 and NF-kappa-B signaling are essential in KRAS mutant tumors, and established a general approach for the rational identification of codependent pathways in cancer.

Uniprot ID

Q9UHD2

RRID

AB_3081037

文献引用格式

TBK1 Antibody (Boster Biological Technology, Wuhan, China. Catalog#A00261-1)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

TANK binding kinase 1

蛋白名全称

Serine/threonine-protein kinase TBK1

实验方案>

实验步骤

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