Western blot (WB): | 1:500-2000 |
Immunohistochemistry (IHC): | 1:50-200 |
Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-200 |
Western blot analysis of anti-TBK1 antibody (BM4038). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: human MCF-7 whole cell lysates,
Lane 4: human K562 whole cell lysates,
Lane 5: rat liver tissue lysates,
Lane 6: rat lung tissue lysates,
Lane 7: mouse liver tissue lysates,
Lane 8: mouse lung tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-TBK1 antigen affinity purified monoclonal antibody (BM4038) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for TBK1 at approximately 84 kDa. The expected band size for TBK1 is at 84 kDa.
Immunohistochemical analysis of paraffin-embedded Rat stomach, using the Antibody.
Immunohistochemical analysis of paraffin-embedded Rat liver, using the Antibody.
Immunohistochemical analysis of paraffin-embedded Mouse skin, using the Antibody.
Immunohistochemical analysis of paraffin-embedded human testis, using NAK/TBK1 (N-term) Antibody.
Immunohistochemical analysis of paraffin-embedded Human lung, using the Antibody.
Immunohistochemical analysis of paraffin-embedded Human colon cancer, using the Antibody.
Immunofluorescent analysis using the Antibody.
Immunofluorescent analysis of MCF7 cells, using NAK/TBK1 (N-term) Antibody.
Western blot analysis of anti-TBK1 antibody (BM4038). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: human MCF-7 whole cell lysates,
Lane 4: human K562 whole cell lysates,
Lane 5: rat liver tissue lysates,
Lane 6: rat lung tissue lysates,
Lane 7: mouse liver tissue lysates,
Lane 8: mouse lung tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-TBK1 antigen affinity purified monoclonal antibody (BM4038) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for TBK1 at approximately 84 kDa. The expected band size for TBK1 is at 84 kDa.
Immunohistochemical analysis of paraffin-embedded Rat stomach, using the Antibody.
Immunohistochemical analysis of paraffin-embedded Rat liver, using the Antibody.
Immunohistochemical analysis of paraffin-embedded Mouse skin, using the Antibody.
Immunohistochemical analysis of paraffin-embedded human testis, using NAK/TBK1 (N-term) Antibody.
Immunohistochemical analysis of paraffin-embedded Human lung, using the Antibody.
Immunohistochemical analysis of paraffin-embedded Human colon cancer, using the Antibody.
Immunofluorescent analysis using the Antibody.
Immunofluorescent analysis of MCF7 cells, using NAK/TBK1 (N-term) Antibody.