Anti-MMP2 Antibody

Rabbit polyclonal antibody

说明书一键复制产品信息

筛选器： All WB ELISA

A00286

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

加入购物车立即购买

Western blot analysis of MMP2 using anti-MMP2 antibody (A00286). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human U87 whole cell lysates,
Lane 2: rat H9C2 whole cell lysates,
Lane 3: mouse 3T3-L1 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-MMP2 antigen affinity purified polyclonal antibody (A00286) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for MMP2 at approximately 72 kDa. The expected band size for MMP2 is at 74 kDa.
all(2)
all(2)

产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-MMP2 Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

72 kDa gelatinase; 72 kDa type IV collagenase; CLG4; CLG4A; Gelatinase A; Matrix metalloproteinase 2; MMP 2; MMP II; MMP2; MMP-2; MONA; TBE 1

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

WB, ELISA

基因名称

MMP2

宿主

Rabbit

抗体亚型

IgG

免疫原

E. coli-derived human MMP2 recombinant protein (Position: K429-C660). Human MMP2 shares 95.7% and 95.3% amino acid (aa) sequence identity with mouse and rat MMP2, respectively.

计算分子量

74 kDa

实际分子量

72 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

Matrix metalloproteinase-2 (MMP2) is a Type IV collagenase, 72-kD, which is also known as gelatinase and is a member of a group of secreted zinc metalloproteases. The MMP2 gene is 17 kb long with 13 exons varying in size from 110 to 901 bp and 12 introns ranging from 175 to 4,350 bp, located within a region of human chromosome 16q13. In addition, the extra exons encode the amino acids of the fibronectin-like domain which has so far been found in only the 72- and 92-kDa type IV collagenase. MMP2, which has a critical role in the binding of progelatinase A and TIMP4 via the C-terminal hemopexin-like domain (C domain), is functionally associated on the surface of angiogenic blood vessels. Not only is a likely effector of endometrial menstrual breakdown, MMP2 is also effector and regulator of the inflammatory response. Moreover, MMP2 could be helpful in diagnosing Takayasu arteritis.

Uniprot ID

P08253

RRID

AB_3081042

文献引用格式

MMP2 Antibody (Boster Biological Technology, Wuhan, China. Catalog#A00286)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

实验方案>

实验步骤

引用文献(0)>

清除