Anti-MMP2 Antibody (Clone#BBA-13)

Rabbit monoclonal antibody

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筛选器： All WB IHC

BM4075

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of anti-MMP2 antibody (BM4075). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-MMP2 antigen affinity purified monoclonal antibody (BM4075) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for MMP2 at approximately 95 kDa. The expected band size for MMP2 is at 74 kDa.
all(2)
Immunohistochemical analysis of paraffin-embedded human breast adenocarcinoma, using MMP2 Antibody.
all(2)

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产品简介>

产品名称

Anti-MMP2 Antibody (Clone#BBA-13)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

72 kDa gelatinase; 72 kDa type IV collagenase; CLG4; CLG4A; Gelatinase A; Matrix metalloproteinase 2; MMP 2; MMP II; MMP2; MMP-2; MONA; TBE 1

产品类型

Monoclonal

检验物种

human, mouse, rat

应用范围

WB, IHC

基因名称

MMP2

克隆号

BBA-13

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthesized peptide derived from human MMP2

计算分子量

74 kDa

实际分子量

72 kDa

成分

500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.

纯化方式

Affinity-chromatography

浓度

500ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.6 months 2 to 8℃ after reconstitution.Avoid repeated freezing and thawing.

背景资料

This gene is a member of the matrix metalloproteinase (MMP) gene family, that are zinc-dependent enzymes capable of cleaving components of the extracellular matrix and molecules involved in signal transduction. The protein encoded by this gene is a gelatinase A, type IV collagenase, that contains three fibronectin type II repeats in its catalytic site that allow binding of denatured type IV and V collagen and elastin. Unlike most MMP family members, activation of this protein can occur on the cell membrane. This enzyme can be activated extracellularly by proteases, or, intracellulary by its S-glutathiolation with no requirement for proteolytical removal of the pro-domain. This protein is thought to be involved in multiple pathways including roles in the nervous system, endometrial menstrual breakdown, regulation of vascularization, and metastasis. Mutations in this gene have been associated with Winchester syndrome and Nodulosis-Arthropathy-Osteolysis (NAO) syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms.

Uniprot ID

P08253

文献引用格式

MMP2 Antibody (Clone#BBA-13) (Boster Biological Technology, Wuhan, China. Catalog#BM4075)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

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