Anti-GSTP1 Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC ICC/IF ELISA

A00394-1

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of GSTP1 using anti-GSTP1 antibody (A00394-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: human Jurkat whole cell lysates,
Lane 4: rat liver tissue lysates,
Lane 5: rat stomach tissue lysates,
Lane 6: mouse liver tissue lysates,
Lane 7: mouse stomach tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-GSTP1 antigen affinity purified polyclonal antibody (A00394-1) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for GSTP1 at approximately 23 kDa. The expected band size for GSTP1 is at 23 kDa.
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IHC analysis of GSTP1 using anti-GSTP1 antibody (A00394-1).
GSTP1 was detected in a paraffin-embedded section of rat kidney tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-GSTP1 Antibody (A00394-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
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IHC analysis of GSTP1 using anti-GSTP1 antibody (A00394-1).
GSTP1 was detected in a paraffin-embedded section of rat liver tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-GSTP1 Antibody (A00394-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
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IF analysis of GSTP1 using anti-GSTP1 antibody (A00394-1).
GSTP1 was detected in an immunocytochemical section of NRK cells. The section was incubated with rabbit anti-GSTP1 Antibody (A00394-1) at a dilution of 1:100. Dylight550-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1135) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
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产品简介>

产品名称

Anti-GSTP1 Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

DFN7; FAEES3; Glutathione S transferase P; glutathione S transferase pi 1; GST class pi; GST3; GSTP1; GSTP1 1; PI

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, ICC/IF, ELISA

基因名称

GSTP1

宿主

Rabbit

抗体亚型

IgG

免疫原

E. coli-derived rat GST3 / GST pi recombinant protein (Position: P2-Q210).

计算分子量

23 kDa

实际分子量

23 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

Glutathione S-transferases pi (GSTP1), also known as GST3, is an enzyme that in humans is encoded by the GSTP1 gene. This gene is mapped to 11q13.2. GSTP1 has 7 exons and 6 introns contained within approximately 2.8 kilobases. GSTP1 belongs to Glutathione S-transferases (GSTs) which are a family of enzymes that play an important role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. GSTP1 presents in all tissues and cells, with the exception of red cells, in which only erythrocyte GST(GSTe) is observed. What's more, GSTP1 is a polymorphic gene encoding active, functionally different GSTP1 variant proteins that are thought to function in xenobiotic metabolism and play a role in susceptibility to cancer, and other diseases.

Uniprot ID

P04906

RRID

AB_3081098

文献引用格式

GSTP1 Antibody (Boster Biological Technology, Wuhan, China. Catalog#A00394-1)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

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