Anti-PPAR Gamma/PPARG Antibody

筛选器： All WB ICC/IF FCM ELISA

• 

  Western blot analysis of anti-PPARG antibody (A00449-3). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human placenta tissue lysates,
  Lane 2: human MCF-7 whole cell lysates,
  Lane 3: human SK-OV-3 whole cell lysates,
  Lane 4: human MDA-MB-453 whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-PPARG antigen affinity purified polyclonal antibody (A00449-3) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for PPARG at approximately 65 kDa. The expected band size for PPARG is at 58 kDa.

• 

  IF analysis of PPARG using anti-PPARG antibody (A00449-3).
  PPARG was detected in an immunocytochemical section of A431 cells. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).

• 

  Flow Cytometry analysis of A431 cells using anti-PPAR Gamma/PPARG antibody (A00449-3).
  Overlay histogram showing A431 cells stained with A00449-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PPAR Gamma/PPARG Antibody (A00449-3) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

• 

  Western blot analysis of anti-PPARG antibody (A00449-3). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human placenta tissue lysates,
  Lane 2: human MCF-7 whole cell lysates,
  Lane 3: human SK-OV-3 whole cell lysates,
  Lane 4: human MDA-MB-453 whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-PPARG antigen affinity purified polyclonal antibody (A00449-3) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for PPARG at approximately 65 kDa. The expected band size for PPARG is at 58 kDa.

• 

  IF analysis of PPARG using anti-PPARG antibody (A00449-3).
  PPARG was detected in an immunocytochemical section of A431 cells. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).

• 

  Flow Cytometry analysis of A431 cells using anti-PPAR Gamma/PPARG antibody (A00449-3).
  Overlay histogram showing A431 cells stained with A00449-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PPAR Gamma/PPARG Antibody (A00449-3) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

•