Anti-PPAR Gamma/PPARG Antibody

Rabbit polyclonal antibody

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筛选器： All WB

BA1693-2

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of PPAR Gamma/PPARG using anti-PPAR Gamma/PPARG antibody (BA1693-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: MM453 whole cell lysates,
Lane 2: MM231 whole cell lysates,
Lane 3: HELA whole cell lysates,
Lane 4: JURKAT whole cell lysates,
Lane 5: HT1080 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-PPAR Gamma/PPARG antigen affinity purified polyclonal antibody (BA1693-2) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for PPAR Gamma/PPARG at approximately 58 kDa. The expected band size for PPAR Gamma/PPARG is at 58 kDa.
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产品名称

Anti-PPAR Gamma/PPARG Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

CIMT1; NR1C3; PPAR gamma; PPARG; PPARG1; PPARG2; PPARgamma; PPARγ

产品类型

Polyclonal

检验物种

human

应用范围

基因名称

PPARG

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence at the N-terminus of human PPAR gamma, different from the rat and mouse sequences by two amino acids.

计算分子量

58 kDa

实际分子量

58 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

The peroxisome proliferator-activated receptors(PPARs) are a group of three nuclear receptor isoforms, PPAR gamma, PPAR alpha, and PPAR delta, encoded by different genes. PPARs are ligand-regulated transcription factors that control gene expression by binding to specific response elements(PPREs) within promoters. PPAR gamma is a transcription factor that has a pivotal role in adipocyte differentiation and expression of adipocyte-specific genes. The PPAR gamma1 and gamma2 isoforms result from alternative splicing and have ligand-dependent and -independent activation domains. PPAR gamma is a member of a family of nuclear receptors/ligand-dependent transcription factors, which bind to hormone response elements on target gene promoters. Ameshima et al.(2003) found that PPAR gamma is abundantly expressed in normal lung tissues, especially in endothelial cells, but that its expression is reduced or absent in the angiogenic plexiform lesions of pulmonary hypertensive lungs and in the vascular lesions of a rat model of severe pulmonary hypertension. And they conclude that fluid shear stress decreases the expression of PPARgamma in endothelial cells and that loss of PPARgamma expression characterizes an abnormal, proliferating, apoptosis-resistant endothelial cell phenotype.

Uniprot ID

P37231

文献引用格式

PPAR Gamma/PPARG Antibody (Boster Biological Technology, Wuhan, China. Catalog#BA1693-2)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

peroxisome proliferator-activated receptor gamma

蛋白名全称

Peroxisome proliferator-activated receptor gamma(PPAR-gamma)

实验方案>

实验步骤

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