Anti-Cyclin E1/CCNE1 Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC FCM ELISA

A00543-2

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of Cyclin E1/CCNE1 using anti-Cyclin E1/CCNE1 antibody (A00543-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: Human HELA whole cell lysates,
Lane 2: Human HepG2 whole cell lysates,
Lane 3: Human K562 whole cell lysates,
Lane 4: Human U2OS whole cell lysates,
Lane 5: Human A549 whole cell lysates,
Lane 6: Human PANC-1 whole cell lysates,
Lane 7: Human CACO-2 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Cyclin E1/CCNE1 antigen affinity purified polyclonal antibody (A00543-2) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Cyclin E1/CCNE1 at approximately 47 kDa. The expected band size for Cyclin E1/CCNE1 is at 47 kDa.
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IHC analysis of Cyclin E1/CCNE1 using anti-Cyclin E1/CCNE1 antibody (A00543-2).
Cyclin E1/CCNE1 was detected in a paraffin-embedded section of human placenta tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Cyclin E1/CCNE1 Antibody (A00543-2) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
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IHC analysis of Cyclin E1/CCNE1 using anti-Cyclin E1/CCNE1 antibody (A00543-2).
Cyclin E1/CCNE1 was detected in a paraffin-embedded section of human placenta tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Cyclin E1/CCNE1 Antibody (A00543-2) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
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IHC analysis of Cyclin E1/CCNE1 using anti-Cyclin E1/CCNE1 antibody (A00543-2).
Cyclin E1/CCNE1 was detected in a paraffin-embedded section of human lung cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Cyclin E1/CCNE1 Antibody (A00543-2) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
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IHC analysis of Cyclin E1/CCNE1 using anti-Cyclin E1/CCNE1 antibody (A00543-2).
Cyclin E1/CCNE1 was detected in a paraffin-embedded section of Mouse testis tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Cyclin E1/CCNE1 Antibody (A00543-2) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
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IHC analysis of Cyclin E1/CCNE1 using anti-Cyclin E1/CCNE1 antibody (A00543-2).
Cyclin E1/CCNE1 was detected in a paraffin-embedded section of rat testis tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Cyclin E1/CCNE1 Antibody (A00543-2) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
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Flow Cytometry analysis of SiHa cells using anti-Cyclin E1/CCNE1 antibody (A00543-2).
Overlay histogram showing SiHa cells stained with A00543-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Cyclin E1/CCNE1 Antibody (A00543-2) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-Cyclin E1/CCNE1 Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

CCNE; CCNE1; cyclin E1; G1/S specific cyclin E1

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, FCM, ELISA

基因名称

CCNE1

宿主

Rabbit

抗体亚型

IgG

免疫原

E.coli-derived human Cyclin E1/CCNE1 recombinant protein (Position: R3-A386).

计算分子量

47 kDa

实际分子量

47 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

G1/S-specific cyclin-E1 is a protein that in humans is encoded by the CCNE1 gene. It is mapped to 19q12. The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition. This protein accumulates at the G1-S phase boundary and is degraded as cells progress through S phase. Overexpression of this gene has been observed in many tumors, which results in chromosome instability, and thus may contribute to tumorigenesis. This protein was found to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a critical role in promoting cell-cycle progression in the absence of pRB.

Uniprot ID

P24864

文献引用格式

Cyclin E1/CCNE1 Antibody (Boster Biological Technology, Wuhan, China. Catalog#A00543-2)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

cyclin E1

蛋白名全称

G1/S-specific cyclin-E1

实验方案>

实验步骤

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