Anti-Cyclin E1/CCNE1 (Phospho-T77) Antibody (Clone#GHC-3)

50μl/1280 100μl/2180 150μl/2800

CCNE; CCNE1; cyclin E1; G1/S specific cyclin E1

Monoclonal

human

WB, IHC, ICC/IF

CCNE1

GHC-3

Rabbit

IgG

A synthesized peptide derived from human Cyclin E1

47 kDa

47 kDa

500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.

Affinity-chromatography

500 ug/ml

Liquid

12 months from date of receipt，-20℃ as supplied.

G1/S-specific cyclin-E1 is a protein that in humans is encoded by the CCNE1 gene. It is mapped to 19q12. The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition. This protein accumulates at the G1-S phase boundary and is degraded as cells progress through S phase. Overexpression of this gene has been observed in many tumors, which results in chromosome instability, and thus may contribute to tumorigenesis. This protein was found to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a critical role in promoting cell-cycle progression in the absence of pRB.

P24864  

Cyclin E1/CCNE1 (Phospho-T77) Antibody (Clone#GHC-3) (Boster Biological Technology, Wuhan, China. Catalog#BM4653)

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

G1/S-specific cyclin-E1

Western blot (WB):	1:500-2000
Immunohistochemistry (IHC):	1:50-200
Immunocytochemistry/Immunofluorescence (ICC/IF):	1:50-200