Anti-SKP2 Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC ICC/IF

A00544

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of SKP2 using anti-SKP2 antibody (A00544). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: human Jurkat whole cell lysates,
Lane 3: human HepG2 whole cell lysates,
Lane 4: human MCF-7 whole cell lysates,
Lane 5: rat testis tissue lysates,
Lane 6: rat C6 whole cell lysates,
Lane 7: mouse testis tissue lysates,
Lane 8: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-SKP2 antigA03957-Aen affinity purified polyclonal antibody (A00544) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for SKP2 at approximately 46, 48 kDa. The expected band size for SKP2 is at 46, 48 kDa.
all(3)
IHC analysis of SKP2 using anti-SKP2 antibody (A00544).
SKP2 was detected in a paraffin-embedded section of human cervical cancer tissue. The tissue section was incubated with rabbit anti-SKP2 Antibody (A00544) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(3)
IF analysis of SKP2 using anti-SKP2 antibody (A00544) and anti-Beta Tubulin antibody (M01857-3).
SKP2 was detected in an immunocytochemical section of Hela cells. The section was incubated with rabbit anti-SKP2 Antibody (A00544) at a dilution of 1:100. Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green) (Catalog # BA1127) and Cy3-conjugated Anti-mouse IgG Secondary Antibody (red) (Catalog # BA1031) were used as secondary antibody.
all(3)

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产品简介>

产品名称

Anti-SKP2 Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

F box protein Skp2; F box/LRR repeat protein 1; FBL1; FBXL1; FLB1; p45skp2; SKP2

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, ICC/IF

基因名称

SKP2

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence at the C-terminus of human SKP2, which shares 88.9% and 94.4% amino acid (aa) sequence identity with mouse and rat SKP2, respectively.

计算分子量

46, 48 kDa

实际分子量

46, 48 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

The F box protein Skp2 (S-phase kinase-associated protein 2) is oncogenic, and its frequent amplification and overexpression correlate with the grade of malignancy of certain tumors. Skp2 controls p300-p53 signaling pathways in cancer cells, making it a potential molecular target for cancer therapy. This gene positively regulates the G(1)-S transition by controlling the stability of several G(1) regulators, such as the cell cycle inhibitor p27. This study provides evidence of a role for an F-box protein in oncogenesis and establishes SKP2 as a protooncogene causally involved in the pathogenesis of lymphomas.

Uniprot ID

Q13309

RRID

AB_3081166

文献引用格式

SKP2 Antibody (Boster Biological Technology, Wuhan, China. Catalog#A00544)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

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