Anti-SKP2 Antibody (Clone#ADCE-19)

Rabbit monoclonal antibody

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筛选器： All WB IHC ICC/IF FCM

BM5389

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of anti-SKP2 antibody (BM5389). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human SIHA whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-SKP2 antigen affinity purified monoclonal antibody (BM5389) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for SKP2 at approximately 46 kDa. The expected band size for SKP2 is at 48 kDa.
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产品简介>

产品名称

Anti-SKP2 Antibody (Clone#ADCE-19)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

F box protein Skp2; F box/LRR repeat protein 1; FBL1; FBXL1; FLB1; p45skp2; SKP2

产品类型

Monoclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, ICC/IF, FCM

基因名称

SKP2

克隆号

ADCE-19

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthesized peptide derived from human SKP2 Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins involved in cell cycle progression, signal transduction and transcription.

计算分子量

48 kDa

实际分子量

48 kDa

成分

500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.

纯化方式

Affinity-chromatography

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

The F box protein Skp2(S-phase kinase-associated protein 2) is oncogenic, and its frequent amplification and overexpression correlate with the grade of malignancy of certain tumors. Skp2 controls p300-p53 signaling pathways in cancer cells, making it a potential molecular target for cancer therapy. This gene positively regulates the G(1)-S transition by controlling the stability of several G(1) regulators, such as the cell cycle inhibitor p27. This study provides evidence of a role for an F-box protein in oncogenesis and establishes SKP2 as a protooncogene causally involved in the pathogenesis of lymphomas.

Uniprot ID

Q13309

文献引用格式

SKP2 Antibody (Clone#ADCE-19) (Boster Biological Technology, Wuhan, China. Catalog#BM5389)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

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