Figure 1. Western blot analysis of Collagen Type III/COL3A1 using anti-Collagen Type III/COL3A1 antibody (A00788-3). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: mouse skin tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Collagen Type III/COL3A1 antigen affinity purified polyclonal antibody (A00788-3) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Collagen Type III/COL3A1 at approximately 200 kDa. The expected band size for Collagen Type III/COL3A1 is at 139 kDa.
Figure 2. IHC analysis of Collagen Type III/COL3A1 using anti-Collagen Type III/COL3A1 antibody (A00788-3) .
Collagen Type III/COL3A1 was detected in a paraffin-embedded section of human gastric poorly differentiated adenocarcinoma tissue. The tissue section was incubated with rabbit anti-Collagen Type III/COL3A1 Antibody (A00788-3) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
Figure 3. IHC analysis of Collagen Type III/COL3A1 using anti-Collagen Type III/COL3A1 antibody (A00788-3) .
Collagen Type III/COL3A1 was detected in a paraffin-embedded section of human tonsil tissue. The tissue section was incubated with rabbit anti-Collagen Type III/COL3A1 Antibody (A00788-3) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
Figure 4. IHC analysis of Collagen Type III/COL3A1 using anti-Collagen Type III/COL3A1 antibody (A00788-3) .
Collagen Type III/COL3A1 was detected in a paraffin-embedded section of human liver cancer tissue. The tissue section was incubated with rabbit anti-Collagen Type III/COL3A1 Antibody (A00788-3) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
Figure 5. IHC analysis of Collagen Type III/COL3A1 using anti-Collagen Type III/COL3A1 antibody (A00788-3) .
Collagen Type III/COL3A1 was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. The tissue section was incubated with rabbit anti-Collagen Type III/COL3A1 Antibody (A00788-3) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
Figure 6. IHC analysis of Collagen Type III/COL3A1 using anti-Collagen Type III/COL3A1 antibody (A00788-3) .
Collagen Type III/COL3A1 was detected in a paraffin-embedded section of mouse lung tissue. The tissue section was incubated with rabbit anti-Collagen Type III/COL3A1 Antibody (A00788-3) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
Figure 7. IHC analysis of Collagen Type III/COL3A1 using anti-Collagen Type III/COL3A1 antibody (A00788-3) .
Collagen Type III/COL3A1 was detected in a paraffin-embedded section of rat lung tissue. The tissue section was incubated with rabbit anti-Collagen Type III/COL3A1 Antibody (A00788-3) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
Figure 8. Flow Cytometry analysis of SiHa cells using anti-Collagen Type III/COL3A1 antibody (A00788-3).
Overlay histogram showing Hela cells stained with A00788-3 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-Collagen Type III/COL3A1 Antibody (A00788-3, 1:100). DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 1:100) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG (Catalog # BA1045) (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
| Western blot (WB): | 1:500-2000 |
| Immunohistochemistry (IHC): | 1:50-400 |
| Flow Cytometry (Fixed): | 1:50-200 |
| Enzyme linked immunosorbent assay (ELISA): | 1:100-1000 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
Figure 1. Western blot analysis of Collagen Type III/COL3A1 using anti-Collagen Type III/COL3A1 antibody (A00788-3). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: mouse skin tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Collagen Type III/COL3A1 antigen affinity purified polyclonal antibody (A00788-3) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Collagen Type III/COL3A1 at approximately 200 kDa. The expected band size for Collagen Type III/COL3A1 is at 139 kDa.
Figure 2. IHC analysis of Collagen Type III/COL3A1 using anti-Collagen Type III/COL3A1 antibody (A00788-3) .
Collagen Type III/COL3A1 was detected in a paraffin-embedded section of human gastric poorly differentiated adenocarcinoma tissue. The tissue section was incubated with rabbit anti-Collagen Type III/COL3A1 Antibody (A00788-3) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
Figure 3. IHC analysis of Collagen Type III/COL3A1 using anti-Collagen Type III/COL3A1 antibody (A00788-3) .
Collagen Type III/COL3A1 was detected in a paraffin-embedded section of human tonsil tissue. The tissue section was incubated with rabbit anti-Collagen Type III/COL3A1 Antibody (A00788-3) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
Figure 4. IHC analysis of Collagen Type III/COL3A1 using anti-Collagen Type III/COL3A1 antibody (A00788-3) .
Collagen Type III/COL3A1 was detected in a paraffin-embedded section of human liver cancer tissue. The tissue section was incubated with rabbit anti-Collagen Type III/COL3A1 Antibody (A00788-3) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
Figure 5. IHC analysis of Collagen Type III/COL3A1 using anti-Collagen Type III/COL3A1 antibody (A00788-3) .
Collagen Type III/COL3A1 was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. The tissue section was incubated with rabbit anti-Collagen Type III/COL3A1 Antibody (A00788-3) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
Figure 6. IHC analysis of Collagen Type III/COL3A1 using anti-Collagen Type III/COL3A1 antibody (A00788-3) .
Collagen Type III/COL3A1 was detected in a paraffin-embedded section of mouse lung tissue. The tissue section was incubated with rabbit anti-Collagen Type III/COL3A1 Antibody (A00788-3) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
Figure 7. IHC analysis of Collagen Type III/COL3A1 using anti-Collagen Type III/COL3A1 antibody (A00788-3) .
Collagen Type III/COL3A1 was detected in a paraffin-embedded section of rat lung tissue. The tissue section was incubated with rabbit anti-Collagen Type III/COL3A1 Antibody (A00788-3) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
Figure 8. Flow Cytometry analysis of SiHa cells using anti-Collagen Type III/COL3A1 antibody (A00788-3).
Overlay histogram showing Hela cells stained with A00788-3 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-Collagen Type III/COL3A1 Antibody (A00788-3, 1:100). DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 1:100) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG (Catalog # BA1045) (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Figure 1. Western blot analysis of Collagen Type III/COL3A1 using anti-Collagen Type III/COL3A1 antibody (A00788-3). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: mouse skin tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Collagen Type III/COL3A1 antigen affinity purified polyclonal antibody (A00788-3) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Collagen Type III/COL3A1 at approximately 200 kDa. The expected band size for Collagen Type III/COL3A1 is at 139 kDa.
Figure 2. IHC analysis of Collagen Type III/COL3A1 using anti-Collagen Type III/COL3A1 antibody (A00788-3) .
Collagen Type III/COL3A1 was detected in a paraffin-embedded section of human gastric poorly differentiated adenocarcinoma tissue. The tissue section was incubated with rabbit anti-Collagen Type III/COL3A1 Antibody (A00788-3) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
Figure 3. IHC analysis of Collagen Type III/COL3A1 using anti-Collagen Type III/COL3A1 antibody (A00788-3) .
Collagen Type III/COL3A1 was detected in a paraffin-embedded section of human tonsil tissue. The tissue section was incubated with rabbit anti-Collagen Type III/COL3A1 Antibody (A00788-3) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
Figure 4. IHC analysis of Collagen Type III/COL3A1 using anti-Collagen Type III/COL3A1 antibody (A00788-3) .
Collagen Type III/COL3A1 was detected in a paraffin-embedded section of human liver cancer tissue. The tissue section was incubated with rabbit anti-Collagen Type III/COL3A1 Antibody (A00788-3) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
Figure 5. IHC analysis of Collagen Type III/COL3A1 using anti-Collagen Type III/COL3A1 antibody (A00788-3) .
Collagen Type III/COL3A1 was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. The tissue section was incubated with rabbit anti-Collagen Type III/COL3A1 Antibody (A00788-3) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
Figure 6. IHC analysis of Collagen Type III/COL3A1 using anti-Collagen Type III/COL3A1 antibody (A00788-3) .
Collagen Type III/COL3A1 was detected in a paraffin-embedded section of mouse lung tissue. The tissue section was incubated with rabbit anti-Collagen Type III/COL3A1 Antibody (A00788-3) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
Figure 7. IHC analysis of Collagen Type III/COL3A1 using anti-Collagen Type III/COL3A1 antibody (A00788-3) .
Collagen Type III/COL3A1 was detected in a paraffin-embedded section of rat lung tissue. The tissue section was incubated with rabbit anti-Collagen Type III/COL3A1 Antibody (A00788-3) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
Figure 8. Flow Cytometry analysis of SiHa cells using anti-Collagen Type III/COL3A1 antibody (A00788-3).
Overlay histogram showing Hela cells stained with A00788-3 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-Collagen Type III/COL3A1 Antibody (A00788-3, 1:100). DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 1:100) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG (Catalog # BA1045) (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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