| Western blot (WB): | 1:500-2000 |
| Enzyme linked immunosorbent assay (ELISA): | 1:100-1000 |

Western blot analysis of TIMELESS using anti-TIMELESS antibody (A00831). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human Jurkat whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-TIMELESS antigen affinity purified polyclonal antibody (A00831) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for TIMELESS at approximately 160 kDa. The expected band size for TIMELESS is at 138 kDa.

Western blot analysis of TIMELESS using anti-TIMELESS antibody (A00831). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human Jurkat whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-TIMELESS antigen affinity purified polyclonal antibody (A00831) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for TIMELESS at approximately 160 kDa. The expected band size for TIMELESS is at 138 kDa.


