Anti-CD34 Antibody

Rabbit polyclonal antibody

说明书

筛选器： All WB IHC ELISA

A00885-1

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

加入购物车立即购买

Figure 1. Western blot analysis of CD34 using anti-CD34 antibody (A00885-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat heart tissue lysates,
Lane 2: rat skeletal muscle tissue lysates,
Lane 3: rat lung tissue lysates,
Lane 4: mouse lung tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-CD34 antigen affinity purified polyclonal antibody (A00885-1) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for CD34 at approximately 105-120 kDa. The expected band size for CD34 is at 105 kDa.
all(4)
Figure 2. IHC analysis of CD34 using anti-CD34 antibody (A00885-1).
CD34 was detected in a paraffin-embedded section of rat brain tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-CD34 Antibody (A00885-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1022) as the chromogen.
all(4)
Figure 3. IHC analysis of CD34 using anti-CD34 antibody (A00885-1).
CD34 was detected in a paraffin-embedded section of rat kidney tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-CD34 Antibody (A00885-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1022) as the chromogen.
all(4)
all(4)

产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-CD34 Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

CD34; CD34 molecule

产品类型

Polyclonal

检验物种

mouse, rat

应用范围

WB, IHC, ELISA

基因名称

CD34

抗体来源

Rabbit

抗体亚型

IgG

免疫原

E.coli-derived rat CD34 recombinant protein (Position: S170-L386).

计算分子量

105 kDa

实际分子量

105-120 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

CD34 is a monomeric cell surface antigen with a molecular mass of approximately 110 KD.CD34 is expressed in humans in hematopoietic stem cells, vascular endothelium, and blasts from 30% of patients with acute myeloid and lymphocytic leukemia. The human CD34 gene spans 26 kb and has 8 exons, a structure quite similar to that of the murine gene. By Southern blot analysis of DNA from a panel of human x mouse somatic cell hybrids using a CD34 cDNA probe demonstrate that the gene for CD34 is located on human chromosome 1 in the 1q12----qter region. CD34 plays an important role in the formation of progenitor cells during both embryonic and adult hematopoiesis.

Uniprot ID

B1PLB1

RRID

AB_3081274

文献引用格式

CD34 Antibody (Boster Biological Technology, Wuhan, China. Catalog#A00885-1)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光; ICC－免疫细胞化学; IHC-F- 冰冻切片免疫组化; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

CD34 molecule

蛋白名全称

Submitted name: CD34 antigen (Predicted)

实验方案>

实验步骤

引用文献(0)>

清除