Figure 1. Western blot analysis of CD34 using anti-CD34 antibody (PB9053). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human placenta tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-CD34 antigen affinity purified polyclonal antibody (PB9053) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for CD34 at approximately 105-120 kDa. The expected band size for CD34 is at 41 kDa.
Figure 2. IHC analysis of CD34 using anti-CD34 antibody (PB9053).
CD34 was detected in a paraffin-embedded section of human lymphoma tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-CD34 Antibody (PB9053) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1022) as the chromogen.
Figure 3. IHC analysis of CD34 using anti-CD34 antibody (PB9053).
CD34 was detected in a paraffin-embedded section of human gastric cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-CD34 Antibody (PB9053) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1022) as the chromogen.
Figure 4. IHC analysis of CD34 using anti-CD34 antibody (PB9053).
CD34 was detected in a paraffin-embedded section of human glioma cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-CD34 Antibody (PB9053) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1022) as the chromogen.
Figure 5. IHC analysis of CD34 using anti-CD34 antibody (PB9053).
CD34 was detected in a paraffin-embedded section of human ovarian cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-CD34 Antibody (PB9053) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1022) as the chromogen.
Figure 6. IHC analysis of CD34 using anti-CD34 antibody (PB9053).
CD34 was detected in a paraffin-embedded section of human placenta tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-CD34 Antibody (PB9053) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1022) as the chromogen.
Figure 7. IHC analysis of CD34 using anti-CD34 antibody (PB9053).
CD34 was detected in a paraffin-embedded section of human liver cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-CD34 Antibody (PB9053) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1022) as the chromogen.
Figure 8. IHC analysis of CD34 using anti-CD34 antibody (PB9053).
CD34 was detected in a paraffin-embedded section of rat kidney tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-CD34 Antibody (PB9053) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1022) as the chromogen.
Figure 9. IF analysis using anti- CD34 antibody (PB9053). detected in paraffin-embedded section of human placenta tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section were stained using the Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green) (Catalog # BA1127) and counterstained with DAPI (blue).
all(11) 
Figure 10. IF analysis using anti- CD34 antibody (PB9053). detected in paraffin-embedded section of human colon cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section were stained using the Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green) (Catalog # BA1127) and counterstained with DAPI (blue).
all(11) 
| Western blot (WB): | 1:500-2000 |
| Immunohistochemistry (IHC): | 1:50-400 |
| Immunofluorescence (IF): | 1:50-400 |
| ELISA： | 1:100-1000 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
Figure 1. Western blot analysis of CD34 using anti-CD34 antibody (PB9053). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human placenta tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-CD34 antigen affinity purified polyclonal antibody (PB9053) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for CD34 at approximately 105-120 kDa. The expected band size for CD34 is at 41 kDa.
Figure 2. IHC analysis of CD34 using anti-CD34 antibody (PB9053).
CD34 was detected in a paraffin-embedded section of human lymphoma tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-CD34 Antibody (PB9053) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1022) as the chromogen.
Figure 3. IHC analysis of CD34 using anti-CD34 antibody (PB9053).
CD34 was detected in a paraffin-embedded section of human gastric cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-CD34 Antibody (PB9053) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1022) as the chromogen.
Figure 4. IHC analysis of CD34 using anti-CD34 antibody (PB9053).
CD34 was detected in a paraffin-embedded section of human glioma cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-CD34 Antibody (PB9053) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1022) as the chromogen.
Figure 5. IHC analysis of CD34 using anti-CD34 antibody (PB9053).
CD34 was detected in a paraffin-embedded section of human ovarian cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-CD34 Antibody (PB9053) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1022) as the chromogen.
Figure 6. IHC analysis of CD34 using anti-CD34 antibody (PB9053).
CD34 was detected in a paraffin-embedded section of human placenta tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-CD34 Antibody (PB9053) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1022) as the chromogen.
Figure 7. IHC analysis of CD34 using anti-CD34 antibody (PB9053).
CD34 was detected in a paraffin-embedded section of human liver cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-CD34 Antibody (PB9053) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1022) as the chromogen.
Figure 8. IHC analysis of CD34 using anti-CD34 antibody (PB9053).
CD34 was detected in a paraffin-embedded section of rat kidney tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-CD34 Antibody (PB9053) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1022) as the chromogen.
Figure 9. IF analysis using anti- CD34 antibody (PB9053). detected in paraffin-embedded section of human placenta tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section were stained using the Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green) (Catalog # BA1127) and counterstained with DAPI (blue).
Figure 10. IF analysis using anti- CD34 antibody (PB9053). detected in paraffin-embedded section of human colon cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section were stained using the Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green) (Catalog # BA1127) and counterstained with DAPI (blue).
Figure 1. Western blot analysis of CD34 using anti-CD34 antibody (PB9053). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human placenta tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-CD34 antigen affinity purified polyclonal antibody (PB9053) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for CD34 at approximately 105-120 kDa. The expected band size for CD34 is at 41 kDa.
Figure 2. IHC analysis of CD34 using anti-CD34 antibody (PB9053).
CD34 was detected in a paraffin-embedded section of human lymphoma tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-CD34 Antibody (PB9053) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1022) as the chromogen.
Figure 3. IHC analysis of CD34 using anti-CD34 antibody (PB9053).
CD34 was detected in a paraffin-embedded section of human gastric cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-CD34 Antibody (PB9053) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1022) as the chromogen.
Figure 4. IHC analysis of CD34 using anti-CD34 antibody (PB9053).
CD34 was detected in a paraffin-embedded section of human glioma cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-CD34 Antibody (PB9053) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1022) as the chromogen.
Figure 5. IHC analysis of CD34 using anti-CD34 antibody (PB9053).
CD34 was detected in a paraffin-embedded section of human ovarian cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-CD34 Antibody (PB9053) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1022) as the chromogen.
Figure 6. IHC analysis of CD34 using anti-CD34 antibody (PB9053).
CD34 was detected in a paraffin-embedded section of human placenta tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-CD34 Antibody (PB9053) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1022) as the chromogen.
Figure 7. IHC analysis of CD34 using anti-CD34 antibody (PB9053).
CD34 was detected in a paraffin-embedded section of human liver cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-CD34 Antibody (PB9053) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1022) as the chromogen.
Figure 8. IHC analysis of CD34 using anti-CD34 antibody (PB9053).
CD34 was detected in a paraffin-embedded section of rat kidney tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-CD34 Antibody (PB9053) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1022) as the chromogen.
Figure 9. IF analysis using anti- CD34 antibody (PB9053). detected in paraffin-embedded section of human placenta tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section were stained using the Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green) (Catalog # BA1127) and counterstained with DAPI (blue).
Figure 10. IF analysis using anti- CD34 antibody (PB9053). detected in paraffin-embedded section of human colon cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section were stained using the Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green) (Catalog # BA1127) and counterstained with DAPI (blue).
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