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Anti-TGF beta 2/TGFB2 Antibody

Rabbit polyclonal antibody

说明书

筛选器: All WB IHC

A00892-1

  • 50μl ¥1280 100μl ¥2180 150μl ¥2800
  • 货期: 现货
  • Figure 1. Western blot analysis of TGF beta 2/TGFB2 using anti-TGF beta 2/TGFB2 antibody (A00892-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
    Lane 1: HCCT whole cell lysates,
    Lane 2: HCCP whole cell lysates.
    After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-TGF beta 2/TGFB2 antigen affinity purified polyclonal antibody (A00892-1) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for TGF beta 2/TGFB2 at approximately 48 kDa. The expected band size for TGF beta 2/TGFB2 is at 48 kDa.

    all(2)
  • Figure 2. IHC analysis of TGF beta 2/TGFB2 using anti-TGF beta 2/TGFB2 antibody (A00892-1).
    TGF beta 2/TGFB2 was detected in a paraffin-embedded section of human lung cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-TGF beta 2/TGFB2 Antibody (A00892-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1022) as the chromogen.

    all(2)

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产品名称
Anti-TGF beta 2/TGFB2 Antibody
规格/价格
50μl/1280 100μl/2180 150μl/2800
指标别称
BSC 1 cell growth inhibitor; Cetermin; G TSF; Polyergin; TGF beta 2; TGF beta2; TGFB2; TGF-beta 2; TGFβ2; TGF-β2
产品类型
Polyclonal
检验物种
human
应用范围
WB, IHC
基因名称
TGFB2
宿主
Rabbit
抗体亚型
IgG
免疫原
A synthetic peptide corresponding to a sequence at the C-terminus of human TGF beta 2, identical to the related mouse and rat sequences.
计算分子量
48 kDa
实际分子量
48 kDa
成分
500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.
纯化方式
Immunogen affinity purified.
浓度
500 ug/ml
产品形态
Liquid
保存条件
12 months from date of receipt,-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.
背景资料
This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate a latency-associated peptide (LAP) and a mature peptide, and is found in either a latent form composed of a mature peptide homodimer, a LAP homodimer, and a latent TGF-beta binding protein, or in an active form consisting solely of the mature peptide homodimer. The mature peptide may also form heterodimers with other TGF-beta family members. Disruption of the TGF-beta/SMAD pathway has been implicated in a variety of human cancers. A chromosomal translocation that includes this gene is associated with Peters' anomaly, a congenital defect of the anterior chamber of the eye. Mutations in this gene may be associated with Loeys-Dietz syndrome. This gene encodes multiple isoforms that may undergo similar proteolytic processing.
Uniprot ID
P61812  
文献引用格式
TGF beta 2/TGFB2 Antibody (Boster Biological Technology, Wuhan, China. Catalog#A00892-1)
应用释义
WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光; ICC-免疫细胞化学; IHC-F- 冰冻切片免疫组化; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;
推荐配套的二抗和检测试剂
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). *Blocking peptide 可以联系我们购买。
推荐稀释比
Western blot (WB):1:500-2000
Immunohistochemistry (IHC):1:50-400
(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user.

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    Anti-TGF beta 2/TGFB2 Antibody

    筛选器: All WB IHC

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