Anti-TGF beta 2/TGFB2 Antibody

Rabbit polyclonal antibody

说明书

筛选器： All WB IHC

A00892-1

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Figure 1. Western blot analysis of TGF beta 2/TGFB2 using anti-TGF beta 2/TGFB2 antibody (A00892-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: HCCT whole cell lysates,
Lane 2: HCCP whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-TGF beta 2/TGFB2 antigen affinity purified polyclonal antibody (A00892-1) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for TGF beta 2/TGFB2 at approximately 48 kDa. The expected band size for TGF beta 2/TGFB2 is at 48 kDa.
all(2)
Figure 2. IHC analysis of TGF beta 2/TGFB2 using anti-TGF beta 2/TGFB2 antibody (A00892-1).
TGF beta 2/TGFB2 was detected in a paraffin-embedded section of human lung cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-TGF beta 2/TGFB2 Antibody (A00892-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1022) as the chromogen.
all(2)

产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-TGF beta 2/TGFB2 Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

BSC 1 cell growth inhibitor; Cetermin; G TSF; Polyergin; TGF beta 2; TGF beta2; TGFB2; TGF-beta 2; TGFβ2; TGF-β2

产品类型

Polyclonal

检验物种

human

应用范围

WB, IHC

基因名称

TGFB2

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence at the C-terminus of human TGF beta 2, identical to the related mouse and rat sequences.

计算分子量

48 kDa

实际分子量

48 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate a latency-associated peptide (LAP) and a mature peptide, and is found in either a latent form composed of a mature peptide homodimer, a LAP homodimer, and a latent TGF-beta binding protein, or in an active form consisting solely of the mature peptide homodimer. The mature peptide may also form heterodimers with other TGF-beta family members. Disruption of the TGF-beta/SMAD pathway has been implicated in a variety of human cancers. A chromosomal translocation that includes this gene is associated with Peters' anomaly, a congenital defect of the anterior chamber of the eye. Mutations in this gene may be associated with Loeys-Dietz syndrome. This gene encodes multiple isoforms that may undergo similar proteolytic processing.

Uniprot ID

P61812

文献引用格式

TGF beta 2/TGFB2 Antibody (Boster Biological Technology, Wuhan, China. Catalog#A00892-1)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光; ICC－免疫细胞化学; IHC-F- 冰冻切片免疫组化; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

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实验步骤

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