Anti-TNFRSF7/CD27 Antibody

Rabbit polyclonal antibody

说明书一键复制产品信息

筛选器： All IHC FCM ELISA

A01148-2

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

加入购物车立即购买

IHC analysis of TNFRSF7/CD27 using anti-TNFRSF7/CD27 antibody (A01148-2).
TNFRSF7/CD27 was detected in a paraffin-embedded section of mouse lymphaden tissue. The tissue section was incubated with rabbit anti-TNFRSF7/CD27 Antibody (A01148-2) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(5)
IHC analysis of TNFRSF7/CD27 using anti-TNFRSF7/CD27 antibody (A01148-2).
TNFRSF7/CD27 was detected in a paraffin-embedded section of rat lymphaden tissue. The tissue section was incubated with rabbit anti-TNFRSF7/CD27 Antibody (A01148-2) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(5)
IHC analysis of TNFRSF7/CD27 using anti-TNFRSF7/CD27 antibody (A01148-2).
TNFRSF7/CD27 was detected in a paraffin-embedded section of rat spleen tissue. The tissue section was incubated with rabbit anti-TNFRSF7/CD27 Antibody (A01148-2) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(5)
Flow Cytometry analysis of mouse PBMC cells using anti-TNFRSF7/CD27 antibody (A01148-2).
Overlay histogram showing mouse PBMC cells stained with A01148-2 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-TNFRSF7/CD27 Antibody (A01148-2) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
all(5)
all(5)

产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-TNFRSF7/CD27 Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

CD27; CD27 antigen; CD27 molecule; CD27L receptor; S152; T cell activation antigen CD27; T14; TNFRSF7; Tp55

产品类型

Polyclonal

检验物种

mouse, rat

应用范围

IHC, FCM, ELISA

基因名称

CD27

宿主

Rabbit

抗体亚型

IgG

免疫原

E.coli-derived mouse Cd27 recombinant protein (Position: P24-F187).

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

CD27 is a member of the tumor necrosis factor receptor superfamily. This receptor is required for generation and long-term maintenance of T cell immunity. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis. And this receptor transduces signals that lead to the activation of NF-kappaB and MAPK8/JNK. Adaptor proteins TRAF2 and TRAF5 have been shown to mediate the signaling process of this receptor. CD27-binding protein (SIVA), a proapoptotic protein, can bind to this receptor and is thought to play an important role in the apoptosis induced by this receptor.

Uniprot ID

P41272

RRID

AB_3081360

文献引用格式

TNFRSF7/CD27 Antibody (Boster Biological Technology, Wuhan, China. Catalog#A01148-2)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

CD27 antigen

蛋白名全称

CD27 antigen

实验方案>

实验步骤

引用文献(0)>

清除