Western blot analysis of TNFRSF7/CD27 using anti-TNFRSF7/CD27 antibody (PB9931). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-TNFRSF7/CD27 antigen affinity purified polyclonal antibody (PB9931) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for TNFRSF7/CD27 at approximately 55 kDa. The expected band size for TNFRSF7/CD27 is at 29 kDa.
Flow Cytometry analysis of A549 cells using anti-CD27 antibody (PB9931).Overlay histogram showing A549 cells stained with PB9931 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CD27 Antibody (PB9931, 1:100) for 30 min at 20°C. DyLight488 conjugated goat anti-rabbit IgG (BA1127, 1:100) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
all(3) 
IF analysis of TNFRSF7/CD27 using anti-TNFRSF7/CD27 antibody (PB9931).
TNFRSF7/CD27 was detected in an immunocytochemical section of K562 cells. The section was incubated with rabbit anti-TNFRSF7/CD27 Antibody (PB9931) at a dilution of 1:100. Dylight550-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1135) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
| Western blot (WB): | 1:500-2000 |
| Immunohistochemistry (IHC): | 1:50-400 |
| Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-400 |
| Flow Cytometry (Fixed): | 1:50-200 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 28 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
Western blot analysis of TNFRSF7/CD27 using anti-TNFRSF7/CD27 antibody (PB9931). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-TNFRSF7/CD27 antigen affinity purified polyclonal antibody (PB9931) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for TNFRSF7/CD27 at approximately 55 kDa. The expected band size for TNFRSF7/CD27 is at 29 kDa.
Flow Cytometry analysis of A549 cells using anti-CD27 antibody (PB9931).Overlay histogram showing A549 cells stained with PB9931 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CD27 Antibody (PB9931, 1:100) for 30 min at 20°C. DyLight488 conjugated goat anti-rabbit IgG (BA1127, 1:100) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
IF analysis of TNFRSF7/CD27 using anti-TNFRSF7/CD27 antibody (PB9931).
TNFRSF7/CD27 was detected in an immunocytochemical section of K562 cells. The section was incubated with rabbit anti-TNFRSF7/CD27 Antibody (PB9931) at a dilution of 1:100. Dylight550-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1135) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
Western blot analysis of TNFRSF7/CD27 using anti-TNFRSF7/CD27 antibody (PB9931). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-TNFRSF7/CD27 antigen affinity purified polyclonal antibody (PB9931) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for TNFRSF7/CD27 at approximately 55 kDa. The expected band size for TNFRSF7/CD27 is at 29 kDa.
Flow Cytometry analysis of A549 cells using anti-CD27 antibody (PB9931).Overlay histogram showing A549 cells stained with PB9931 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CD27 Antibody (PB9931, 1:100) for 30 min at 20°C. DyLight488 conjugated goat anti-rabbit IgG (BA1127, 1:100) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
IF analysis of TNFRSF7/CD27 using anti-TNFRSF7/CD27 antibody (PB9931).
TNFRSF7/CD27 was detected in an immunocytochemical section of K562 cells. The section was incubated with rabbit anti-TNFRSF7/CD27 Antibody (PB9931) at a dilution of 1:100. Dylight550-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1135) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
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