Western blot analysis of CD9 using anti-CD9 antibody (A01202-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: HCCT tissue lysates,
Lane 2: RT4 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-CD9 antigen affinity purified polyclonal antibody (A01202-2) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for CD9 at approximately 25 kDa. The expected band size for CD9 is at 25 kDa.
IHC analysis of CD9 using anti-CD9 antibody (A01202-2).
CD9 was detected in a paraffin-embedded section of human lung cancer tissue. The tissue section was incubated with rabbit anti-CD9 Antibody (A01202-2) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of CD9 using anti-CD9 antibody (A01202-2).
CD9 was detected in a paraffin-embedded section of human adenocarcinoma of the right colon tissue. The tissue section was incubated with rabbit anti-CD9 Antibody (A01202-2) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IF analysis using anti- CD9 Antibody (A01202-2). detected in paraffin-embedded section of human intestine cancer tissue. The tissue section were stained using the cy3-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog # BA1032). Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green) (Catalog # BA1127) and counterstained with DAPI (blue).
all(6) 
Flow Cytometry analysis of Jurkat cells using anti-CD9 antibody (A01202-2).
Overlay histogram showing Jurkat cells stained with A01202-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CD9 Antibody (A01202-2) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
| Western blot (WB): | 1:500-2000 |
| Immunohistochemistry (IHC): | 1:50-400 |
| Immunofluorescence (IF): | 1:50-400 |
| Flow Cytometry (Fixed): | 1:50-200 |
| Enzyme linked immunosorbent assay (ELISA): | 1:100-1000 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
Western blot analysis of CD9 using anti-CD9 antibody (A01202-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: HCCT tissue lysates,
Lane 2: RT4 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-CD9 antigen affinity purified polyclonal antibody (A01202-2) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for CD9 at approximately 25 kDa. The expected band size for CD9 is at 25 kDa.
IHC analysis of CD9 using anti-CD9 antibody (A01202-2).
CD9 was detected in a paraffin-embedded section of human lung cancer tissue. The tissue section was incubated with rabbit anti-CD9 Antibody (A01202-2) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of CD9 using anti-CD9 antibody (A01202-2).
CD9 was detected in a paraffin-embedded section of human adenocarcinoma of the right colon tissue. The tissue section was incubated with rabbit anti-CD9 Antibody (A01202-2) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IF analysis using anti- CD9 Antibody (A01202-2). detected in paraffin-embedded section of human intestine cancer tissue. The tissue section were stained using the cy3-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog # BA1032). Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green) (Catalog # BA1127) and counterstained with DAPI (blue).
Flow Cytometry analysis of Jurkat cells using anti-CD9 antibody (A01202-2).
Overlay histogram showing Jurkat cells stained with A01202-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CD9 Antibody (A01202-2) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Western blot analysis of CD9 using anti-CD9 antibody (A01202-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: HCCT tissue lysates,
Lane 2: RT4 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-CD9 antigen affinity purified polyclonal antibody (A01202-2) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for CD9 at approximately 25 kDa. The expected band size for CD9 is at 25 kDa.
IHC analysis of CD9 using anti-CD9 antibody (A01202-2).
CD9 was detected in a paraffin-embedded section of human lung cancer tissue. The tissue section was incubated with rabbit anti-CD9 Antibody (A01202-2) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of CD9 using anti-CD9 antibody (A01202-2).
CD9 was detected in a paraffin-embedded section of human adenocarcinoma of the right colon tissue. The tissue section was incubated with rabbit anti-CD9 Antibody (A01202-2) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IF analysis using anti- CD9 Antibody (A01202-2). detected in paraffin-embedded section of human intestine cancer tissue. The tissue section were stained using the cy3-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog # BA1032). Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green) (Catalog # BA1127) and counterstained with DAPI (blue).
Flow Cytometry analysis of Jurkat cells using anti-CD9 antibody (A01202-2).
Overlay histogram showing Jurkat cells stained with A01202-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CD9 Antibody (A01202-2) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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