Anti-CD9 Antibody (Clone#CED-3)

Rabbit monoclonal antibody

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筛选器： All WB IHC ICC/IF IP FCM

BM4212

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of anti-CD9 antibody (BM4212). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hacat whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human A549 whole cell lysates,
Lane 4: mouse heart tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-CD9 antigen affinity purified monoclonal antibody (BM4212) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for CD9 at approximately 23 kDa. The expected band size for CD9 is at 25 kDa.
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IHC analysis of CD9 using anti-CD9 antibody (BM4212) .
CD9 was detected in a paraffin-embedded section of human colon cancer tissue. The tissue section was incubated with rabbit anti-CD9 Antibody (BM4212) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
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IHC analysis of CD9 using anti-CD9 antibody (BM4212) .
CD9 was detected in a paraffin-embedded section of human prostate cancer tissue. The tissue section was incubated with rabbit anti-CD9 Antibody (BM4212) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
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IHC analysis of CD9 using anti-CD9 antibody (BM4212) .
CD9 was detected in a paraffin-embedded section of mouse brain tissue. The tissue section was incubated with rabbit anti-CD9 Antibody (BM4212) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
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IHC analysis of CD9 using anti-CD9 antibody (BM4212) .
CD9 was detected in a paraffin-embedded section of rat brain tissue. The tissue section was incubated with rabbit anti-CD9 Antibody (BM4212) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
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Immunofluorescent analysis of Hela cells, using CD9 Antibody .
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产品名称

Anti-CD9 Antibody (Clone#CED-3)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

5H9; 5H9 antigen; BA2; BTCC 1; CD9; CD9 antigen; CD9 molecule; Cell and organelle markers; DRAP 27; Exosome marker; GIG2; Leukocyte antigen MIC3; MIC3; Motility related protein; MRP 1; P24; Tetraspanin 29; Tspan 29; TSPAN29

产品类型

Monoclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, ICC/IF, IP, FCM

基因名称

CD9

克隆号

CED-3

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthesized peptide derived from human CD9

计算分子量

25 kDa

实际分子量

23 kDa

成分

500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.

纯化方式

Affinity-chromatography

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

CD9 antigen is a protein that in humans is encoded by the CD9 gene. CD9 is a cell surface glycoprotein that is known to complex with integrins and other transmembrane 4 superfamily proteins. It is found on the surface of exosomes. It can modulate cell adhesion and migration and also trigger platelet activation and aggregation. In addition, the protein appears to promote muscle cell fusion and support myotube maintenance. This protein also seems to be a key part in the egg-sperm fusion during mammalian fertilization. While oocytes are ovulated, CD9-deficient oocytes are not properly fused with sperm upon fertilization. CD9 is located in the microvillar membrane of the oocytes and also appears to intervene in maintaining the normal shape of oocyte microvilli.

Uniprot ID

P21926

文献引用格式

CD9 Antibody (Clone#CED-3) (Boster Biological Technology, Wuhan, China. Catalog#BM4212)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

CD9 antigen

实验方案>

实验步骤

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