Immunohistochemistry (IHC): | 1:50-400 |
Immunofluorescence (IF): | 1:50-400 |
Enzyme linked immunosorbent assay (ELISA): | 1:100-1000 |
(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. |
Figure 1. IHC analysis of MUC2 using anti-MUC2 antibody (A01212-2).
MUC2 was detected in a paraffin-embedded section of human colon cancer tissue. The tissue section was incubated with rabbit anti-MUC2 Antibody (A01212-2) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
Figure 2. IF analysis of MUC2 using anti-MUC2 antibody (A01212-2).
MUC2 was detected in a paraffin-embedded section of human colon cancer tissue. The tissue section was incubated with rabbit anti-MUC2 Antibody (A01212-2) at a dilution of 1:100. Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green)(Catalog#BA1127) was used as secondary antibody.
Figure 1. IHC analysis of MUC2 using anti-MUC2 antibody (A01212-2).
MUC2 was detected in a paraffin-embedded section of human colon cancer tissue. The tissue section was incubated with rabbit anti-MUC2 Antibody (A01212-2) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
Figure 2. IF analysis of MUC2 using anti-MUC2 antibody (A01212-2).
MUC2 was detected in a paraffin-embedded section of human colon cancer tissue. The tissue section was incubated with rabbit anti-MUC2 Antibody (A01212-2) at a dilution of 1:100. Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green)(Catalog#BA1127) was used as secondary antibody.