Western blot analysis of ENO1 using anti-ENO1 antibody (A01250-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HELA whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human SH-SY5Y whole cell lysates,
Lane 4: human U-87MG whole cell lysates,
Lane 5: human HEK293 whole cell lysates,
Lane 6: human CACO-2 whole cell lysates,
Lane 7: Monkey kidney tissue lysates,
Lane 8: Monkey liver tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ENO1 antigen affinity purified polyclonal antibody (A01250-1) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for ENO1 at approximately 47 kDa. The expected band size for ENO1 is at 47 kDa.
Western blot analysis of ENO1 using anti-ENO1 antibody (A01250-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: Rat brain tissue lysates,
Lane 2: Rat heart tissue lysates,
Lane 3: Rat kidney tissue lysates,
Lane 4: Rat liver tissue lysates,
Lane 5: Mouse brain tissue lysates,
Lane 6: Mouse kidney tissue lysates,
Lane 7: Mouse liver tissue lysates,
Lane 8: Mouse RAW264.7 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ENO1 antigen affinity purified polyclonal antibody (A01250-1) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for ENO1 at approximately 47 kDa. The expected band size for ENO1 is at 47 kDa.
IHC analysis of ENO1 using anti-ENO1 antibody (A01250-1).
ENO1 was detected in a paraffin-embedded section of human liver cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-ENO1 Antibody (A01250-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of ENO1 using anti-ENO1 antibody (A01250-1).
ENO1 was detected in a paraffin-embedded section of mouse testis tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-ENO1 Antibody (A01250-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of ENO1 using anti-ENO1 antibody (A01250-1).
ENO1 was detected in a paraffin-embedded section of rat testis tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-ENO1 Antibody (A01250-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
Flow Cytometry analysis of HL-60 cells using anti-ENO1 antibody (A01250-1).
Overlay histogram showing HL-60 cells stained with A01250-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ENO1 Antibody (A01250-1) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
IF analysis of ENO1 using anti-ENO1 antibody (A01250-1).
ENO1 was detected in an immunocytochemical section of A431 cells. The section was incubated with rabbit anti-ENO1 Antibody (A01250-1) at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
IP analysis of ENO1 using anti-ENO1 antibody (A01250-1) in A549 whole cell lysate.
Western blot analysis of ENO1 using anti- ENO1 antibody (A01250-1).
Lane 1: A549 whole cell lysates(30ug),
Lane 2: Rabbit control IgG instead of anti- ENO1 antibody in A549 whole cell lysate,
Lane 3: anti- ENO1 antibody (2μg) + A549 whole cell lysate (500μg).
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti- ENO1 antigen affinity purified polyclonal antibody (A01250-1) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for ENO1 at approximately 47 kDa. The expected band size for ENO1 is at 47 kDa.
| Western blot (WB): | 1:500-2000 |
| Immunohistochemistry (IHC): | 1:50-400 |
| Immunocytochemistry/Immunofluorescence(ICC/IF): | 1:50-400 |
| ImmunoPrecipitation (IP): | 1:250-300 |
| Flow Cytometry (Fixed): | 1:50-200 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
Western blot analysis of ENO1 using anti-ENO1 antibody (A01250-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HELA whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human SH-SY5Y whole cell lysates,
Lane 4: human U-87MG whole cell lysates,
Lane 5: human HEK293 whole cell lysates,
Lane 6: human CACO-2 whole cell lysates,
Lane 7: Monkey kidney tissue lysates,
Lane 8: Monkey liver tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ENO1 antigen affinity purified polyclonal antibody (A01250-1) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for ENO1 at approximately 47 kDa. The expected band size for ENO1 is at 47 kDa.
Western blot analysis of ENO1 using anti-ENO1 antibody (A01250-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: Rat brain tissue lysates,
Lane 2: Rat heart tissue lysates,
Lane 3: Rat kidney tissue lysates,
Lane 4: Rat liver tissue lysates,
Lane 5: Mouse brain tissue lysates,
Lane 6: Mouse kidney tissue lysates,
Lane 7: Mouse liver tissue lysates,
Lane 8: Mouse RAW264.7 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ENO1 antigen affinity purified polyclonal antibody (A01250-1) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for ENO1 at approximately 47 kDa. The expected band size for ENO1 is at 47 kDa.
IHC analysis of ENO1 using anti-ENO1 antibody (A01250-1).
ENO1 was detected in a paraffin-embedded section of human liver cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-ENO1 Antibody (A01250-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of ENO1 using anti-ENO1 antibody (A01250-1).
ENO1 was detected in a paraffin-embedded section of mouse testis tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-ENO1 Antibody (A01250-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of ENO1 using anti-ENO1 antibody (A01250-1).
ENO1 was detected in a paraffin-embedded section of rat testis tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-ENO1 Antibody (A01250-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
Flow Cytometry analysis of HL-60 cells using anti-ENO1 antibody (A01250-1).
Overlay histogram showing HL-60 cells stained with A01250-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ENO1 Antibody (A01250-1) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
IF analysis of ENO1 using anti-ENO1 antibody (A01250-1).
ENO1 was detected in an immunocytochemical section of A431 cells. The section was incubated with rabbit anti-ENO1 Antibody (A01250-1) at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
IP analysis of ENO1 using anti-ENO1 antibody (A01250-1) in A549 whole cell lysate.
Western blot analysis of ENO1 using anti- ENO1 antibody (A01250-1).
Lane 1: A549 whole cell lysates(30ug),
Lane 2: Rabbit control IgG instead of anti- ENO1 antibody in A549 whole cell lysate,
Lane 3: anti- ENO1 antibody (2μg) + A549 whole cell lysate (500μg).
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti- ENO1 antigen affinity purified polyclonal antibody (A01250-1) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for ENO1 at approximately 47 kDa. The expected band size for ENO1 is at 47 kDa.
Western blot analysis of ENO1 using anti-ENO1 antibody (A01250-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HELA whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human SH-SY5Y whole cell lysates,
Lane 4: human U-87MG whole cell lysates,
Lane 5: human HEK293 whole cell lysates,
Lane 6: human CACO-2 whole cell lysates,
Lane 7: Monkey kidney tissue lysates,
Lane 8: Monkey liver tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ENO1 antigen affinity purified polyclonal antibody (A01250-1) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for ENO1 at approximately 47 kDa. The expected band size for ENO1 is at 47 kDa.
Western blot analysis of ENO1 using anti-ENO1 antibody (A01250-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: Rat brain tissue lysates,
Lane 2: Rat heart tissue lysates,
Lane 3: Rat kidney tissue lysates,
Lane 4: Rat liver tissue lysates,
Lane 5: Mouse brain tissue lysates,
Lane 6: Mouse kidney tissue lysates,
Lane 7: Mouse liver tissue lysates,
Lane 8: Mouse RAW264.7 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ENO1 antigen affinity purified polyclonal antibody (A01250-1) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for ENO1 at approximately 47 kDa. The expected band size for ENO1 is at 47 kDa.
IHC analysis of ENO1 using anti-ENO1 antibody (A01250-1).
ENO1 was detected in a paraffin-embedded section of human liver cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-ENO1 Antibody (A01250-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of ENO1 using anti-ENO1 antibody (A01250-1).
ENO1 was detected in a paraffin-embedded section of mouse testis tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-ENO1 Antibody (A01250-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of ENO1 using anti-ENO1 antibody (A01250-1).
ENO1 was detected in a paraffin-embedded section of rat testis tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-ENO1 Antibody (A01250-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
Flow Cytometry analysis of HL-60 cells using anti-ENO1 antibody (A01250-1).
Overlay histogram showing HL-60 cells stained with A01250-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ENO1 Antibody (A01250-1) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
IF analysis of ENO1 using anti-ENO1 antibody (A01250-1).
ENO1 was detected in an immunocytochemical section of A431 cells. The section was incubated with rabbit anti-ENO1 Antibody (A01250-1) at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
IP analysis of ENO1 using anti-ENO1 antibody (A01250-1) in A549 whole cell lysate.
Western blot analysis of ENO1 using anti- ENO1 antibody (A01250-1).
Lane 1: A549 whole cell lysates(30ug),
Lane 2: Rabbit control IgG instead of anti- ENO1 antibody in A549 whole cell lysate,
Lane 3: anti- ENO1 antibody (2μg) + A549 whole cell lysate (500μg).
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti- ENO1 antigen affinity purified polyclonal antibody (A01250-1) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for ENO1 at approximately 47 kDa. The expected band size for ENO1 is at 47 kDa.
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