Anti-ENO1 Antibody (Clone#AEA-5)

Rabbit monoclonal antibody

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筛选器： All WB IHC ICC/IF IP

BM4004

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of anti-ENO1 antibody (BM4004). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human MCF-7 whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human U251 whole cell lysates,
Lane 4: human A549 whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat testis tissue lysates,
Lane 7: mouse brain tissue lysates,
Lane 8: mouse testis tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ENO1 antigen affinity purified monoclonal antibody (BM4004) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for ENO1 at approximately 47 kDa. The expected band size for ENO1 is at 47 kDa.
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Immunohistochemical analysis of paraffin-embedded human colon, using ENO1 Antibody.
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产品简介>

产品名称

Anti-ENO1 Antibody (Clone#AEA-5)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

Alpha enolase; C myc promoter binding protein; ENO1; ENO1L1; Enolase 1; enolase 1; (alpha); MBP 1; MBPB1; MPB 1; MPB1; NNE; Non neural enolase; Phosphopyruvate hydratase; Plasminogen binding protein; PPH

产品类型

Monoclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, ICC/IF, IP

基因名称

ENO1

克隆号

AEA-5

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthesized peptide derived from human ENO1

计算分子量

47 kDa

实际分子量

47 kDa

成分

500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.

纯化方式

Affinity-chromatography

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

Enolase 1 (ENO1) is a glycolytic enzyme expressed in most tissues. It is mapped to 1p36.23. This gene encodes alpha-enolase, one of three enolase isoenzymes found in mammals. Each isoenzyme is a homodimer composed of 2 alpha, 2 gamma, or 2 beta subunits, and functions as a glycolytic enzyme. Alpha-enolase in addition, functions as a structural lens protein (tau-crystallin) in the monomeric form. Alternative splicing of this gene results in a shorter isoform that has been shown to bind to the c-myc promoter and function as a tumor suppressor. Several pseudogenes have been identified, including one on the long arm of chromosome 1. Alpha-enolase has also been identified as an autoantigen in Hashimoto encephalopathy.

Uniprot ID

P06733

文献引用格式

ENO1 Antibody (Clone#AEA-5) (Boster Biological Technology, Wuhan, China. Catalog#BM4004)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

Alpha-enolase

实验方案>

实验步骤

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