Anti-CYP27B1 Antibody

Rabbit polyclonal antibody

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筛选器： All WB ELISA

A01370

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of anti-CYP27B1 antibody (A01370). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-CYP27B1 antigen affinity purified polyclonal antibody (A01370) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for CYP27B1 at approximately 57 kDa. The expected band size for CYP27B1 is at 57 kDa.
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产品简介>

产品名称

Anti-CYP27B1 Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

cytochrome P450 family 27 subfamily B member 1; CP2B; CYP1; CYP1alpha; CYP27B; P450c1; PDDR; VDD1; VDDR; VDDRI; VDR; CYP27B1

产品类型

Polyclonal

检验物种

human

应用范围

WB, ELISA

基因名称

CYP27B1

宿主

Rabbit

抗体亚型

IgG

免疫原

E.coli-derived human CYP27B1 recombinant protein (Position: R252-R508).

计算分子量

57 kDa

实际分子量

57 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

CYP27B1 belongs to the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. The protein encoded by this gene localizes to the inner mitochondrial membrane where it hydroxylates 25-hydroxyvitamin D3 at the 1alpha position. This reaction synthesizes 1alpha,25-dihydroxyvitamin D3, the active form of vitamin D3, which binds to the vitamin D receptor and regulates calcium metabolism. Thus this enzyme regulates the level of biologically active vitamin D and plays an important role in calcium homeostasis. Mutations in this gene can result in vitamin D-dependent rickets type I.

Uniprot ID

O15528

Gene ID

1594

文献引用格式

CYP27B1 Antibody (Boster Biological Technology, Wuhan, China. Catalog#A01370)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

cytochrome P450 family 27 subfamily B member 1

蛋白名全称

25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial

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实验步骤

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