Anti-CYP27B1 Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC

PB0577

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of anti- CYP27B1 antibody (PB0577). The sample well of each lane was loaded with 30ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human Caco-2 whole cell lysates,
Lane 3: rat heart tissue lysates,
Lane 4: rat brain tissue lysates,
Lane 5: mouse heart tissue lysates,
Lane 6: mouse RAW264.7 whole cell lysates.
Use rabbit anti- CYP27B1 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog#EK1002). A specific band was detected for CYP27B1 at approximately 57KD. The expected band size for CYP27B1 is at 57KD.
all(3)
IHC analysis of CYP27B1 using anti-CYP27B1 antibody (PB0577).
CYP27B1 was detected in a paraffin-embedded section of rat kidney tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-CYP27B1 Antibody (PB0577) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(3)
IHC analysis of CYP27B1 using anti-CYP27B1 antibody (PB0577).
CYP27B1 was detected in a paraffin-embedded section of Human Renal Cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-CYP27B1 Antibody (PB0577) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(3)

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产品简介>

产品名称

Anti-CYP27B1 Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

cytochrome P450 family 27 subfamily B member 1; CP2B; CYP1; CYP1alpha; CYP27B; P450c1; PDDR; VDD1; VDDR; VDDRI; VDR; CYP27B1

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

WB, IHC

基因名称

CYP27B1

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence at the C-terminus of human CYP27B1 different from the related mouse and rat sequences by six amino acids.

计算分子量

57 kDa

实际分子量

57 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

CYP27B1 belongs to the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. The protein encoded by this gene localizes to the inner mitochondrial membrane where it hydroxylates 25-hydroxyvitamin D3 at the 1alpha position. This reaction synthesizes 1alpha,25-dihydroxyvitamin D3, the active form of vitamin D3, which binds to the vitamin D receptor and regulates calcium metabolism. Thus this enzyme regulates the level of biologically active vitamin D and plays an important role in calcium homeostasis. Mutations in this gene can result in vitamin D-dependent rickets type I.

Uniprot ID

O15528

文献引用格式

CYP27B1 Antibody (Boster Biological Technology, Wuhan, China. Catalog#PB0577)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

cytochrome P450, family 27, subfamily B, polypeptide 1

蛋白名全称

25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial

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