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Anti-TPX2 Antibody

Rabbit polyclonal antibody

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筛选器: All WB ELISA

A01610-1

  • 50μl ¥1280 100μl ¥2180 150μl ¥2800
  • 货期: 现货
  • Western blot analysis of TPX2 using anti-TPX2 antibody (A01610-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
    Lane 1: human Hela whole cell lysates,
    Lane 2: human COLO-320 whole cell lysates,
    Lane 3: human U-87MG whole cell lysates,
    Lane 4: human SGC-7901 whole cell lysates,
    Lane 5: rat PC-12 whole cell lysates,
    Lane 6: mouse HEPA1-6 whole cell lysates.
    After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-TPX2 antigen affinity purified polyclonal antibody (A01610-1) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for TPX2 at approximately 100 kDa. The expected band size for TPX2 is at 86 kDa.

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产品名称
Anti-TPX2 Antibody
规格/价格
50μl/1280 100μl/2180 150μl/2800
指标别称
C20orf1; C20orf2; DIL 2; DIL2; FLS353; GD:C20orf1; HCA519; HCTP4; p100; Protein fls353; REPP86; Targeting protein for Xklp2; TPX2
产品类型
Polyclonal
检验物种
human, mouse, rat
应用范围
WB, ELISA
基因名称
TPX2
宿主
Rabbit
抗体亚型
IgG
免疫原
E. coli-derived human TPX2 recombinant protein (Position: D15-Q93).
计算分子量
86 kDa
实际分子量
100 kDa
成分
500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.
纯化方式
Immunogen affinity purified.
浓度
500 ug/ml
产品形态
Liquid
保存条件
12 months from date of receipt,-20℃ as supplied.
背景资料
Targeting protein for Xklp2, also known as C20ORF1, is a protein that in humans is encoded by the TPX2 gene. It is mapped to chromosome 20q11.2. It was concluded that even in the presence of duplicated centrosomes, spindle formation requires the function of TPX2 to generate a stable bipolar spindle with overlapping antiparallel microtubule arrays. At the onset of mitosis, GOLGA2 interacts with importin-alpha, liberating TPX2 from importin-alpha, allowing TPX2 to activates AURKA kinase and stimulates local microtubule nucleation.
Uniprot ID
Q9ULW0  
文献引用格式
TPX2 Antibody (Boster Biological Technology, Wuhan, China. Catalog#A01610-1)
应用释义
WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;
推荐配套的二抗和检测试剂
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). *Blocking peptide 可以联系我们购买。
推荐稀释比
Western blot (WB):1:500-2000
ELISA:1:100-1000

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    Anti-TPX2 Antibody

    筛选器: All WB ELISA

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