Anti-2B4/CD244 Antibody

Rabbit polyclonal antibody

说明书

筛选器： All WB ELISA

A02527-1

50μl ￥1180 100μl ￥1960 150μl ￥2780
货期：现货

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Figure 1. Western blot analysis of 2B4/CD244 using anti-2B4/CD244 antibody (A02527-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: mouse spleen tissue lysates,
Lane 2: mouse thymus tissue lysates,
Lane 3: mouse RAW264.7 whole cell lysates,
Lane 3: mouse SP20 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-2B4/CD244 antigen affinity purified polyclonal antibody (A02527-1) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for 2B4/CD244 at approximately 42 kDa. The expected band size for 2B4/CD244 is at 42 kDa.
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产品简介>

产品名称

Anti-2B4/CD244 Antibody

规格/价格

50μl/1180 100μl/1960 150μl/2780

指标别称

2B4; CD244; h2B4; NAIL; NKR2B4; SLAMF4

产品类型

Polyclonal

检验物种

mouse

应用范围

WB, ELISA

基因名称

CD244

抗体来源

Rabbit

抗体亚型

IgG

免疫原

E.coli-derived mouse 2B4/Cd244 recombinant protein (Position: Q20-N374).

计算分子量

42 kDa

实际分子量

42 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

CD244(Cluster of Differentiation 244) is a human protein encoded by the CD244 gene. It is also known as Natural Killer Cell Receptor 2B4. Tangye et al.(1999) mapped the 2B4 gene to 1q22. Suzuki et al.(2008) identified a functional single-nucleotide polymorphism(SNP) in the CD244 gene that contributes to rheumatoid arthritis susceptibility. Functional analysis by Boles et al.(1999) demonstrated that engagement of 2B4 with specific antibody activates NK cytolytic activity. Using recombinant human NK cell-activating ligand 2B4 fused to domains 3 and 4 of rodent Cd4 and flow cytometric analysis, Brown et al.(1998) demonstrated that CD48 binds to 2B4. Watzl et al.(2000) showed that antibody-mediated cross-linking of 2B4 leads to its rapid tyrosine phosphorylation, which is necessary for 2B4-mediated killer cell activity.

Uniprot ID

Q07763

文献引用格式

2B4/CD244 Antibody (Boster Biological Technology, Wuhan, China. Catalog#A02527-1)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光; ICC－免疫细胞化学; IHC-F- 冰冻切片免疫组化; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

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