| Western blot (WB): | 1:500-2000 |
| Enzyme linked immunosorbent assay (ELISA): | 1:100-1000 |

Western blot analysis of anti-Caspr2/CNTNAP2 antibody (A02819-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Caspr2/CNTNAP2 antigen affinity purified polyclonal antibody (A02819-2) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Caspr2/CNTNAP2 at approximately 170 kDa. The expected band size for Caspr2/CNTNAP2 is at 170 kDa.

Western blot analysis of anti-Caspr2/CNTNAP2 antibody (A02819-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Caspr2/CNTNAP2 antigen affinity purified polyclonal antibody (A02819-2) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Caspr2/CNTNAP2 at approximately 170 kDa. The expected band size for Caspr2/CNTNAP2 is at 170 kDa.


