Anti-ADAM9 Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC ICC/IF ELISA

A03074-1

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of ADAM9 using anti-ADAM9 antibody (A03074-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: U-87MG whole cell lysates,
Lane 2: A431 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ADAM9 antigen affinity purified polyclonal antibody (A03074-1) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for ADAM9 at approximately 75 kDa/100 kDa. The expected band size for ADAM9 is at 91 kDa.
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IHC analysis of ADAM9 using anti-ADAM9 antibody (A03074-1).
ADAM9 was detected in a paraffin-embedded section of human bladder adenosquamous carcinomahuman tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-ADAM9 Antibody (A03074-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
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IF analysis of ADAM9 using anti-ADAM9 antibody (A03074-1).
ADAM9 was detected in an immunocytochemical section of U2OS cells. The section was incubated with rabbit anti-ADAM9 Antibody (A03074-1) at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
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产品简介>

产品名称

Anti-ADAM9 Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

ADAM 9; Cellular disintegrin-related protein; Meltrin-gamma; Myeloma cell metalloproteinase; ADAM9; KIAA0021; MCMP; MDC9; MLTNG; CORD9

产品类型

Polyclonal

检验物种

human

应用范围

WB, IHC, ICC/IF, ELISA

基因名称

ADAM9

宿主

Rabbit

抗体亚型

IgG

免疫原

E.coli-derived human ADAM9 recombinant protein (Position: R212-D247).

计算分子量

91 kDa

实际分子量

75 kDa/100 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

Disintegrin and metalloproteinase domain-containing protein 9 is an enzyme that in humans is encoded by the ADAM9 gene. This gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. The protein encoded by this gene interacts with SH3 domain-containing proteins, binds mitotic arrest deficient 2 beta protein, and is also involved in TPA-induced ectodomain shedding of membrane-anchored heparin-binding EGF-like growth factor. Several alternatively spliced transcript variants have been identified for this gene.

Uniprot ID

Q13443

文献引用格式

ADAM9 Antibody (Boster Biological Technology, Wuhan, China. Catalog#A03074-1)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光; ICC－免疫细胞化学; IHC-F- 冰冻切片免疫组化; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

ADAM metallopeptidase domain 9

蛋白名全称

Disintegrin and metalloproteinase domain-containing protein 9

实验方案>

实验步骤

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