Anti-CPT1B Antibody

筛选器： All WB ICC/IF FCM ELISA

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  Western blot analysis of CPT1B using anti-CPT1B antibody (A03558). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: monkey heart tissue lysates,
  Lane 2: rat heart tissue lysates,
  Lane 3: mouse heart tissue lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-CPT1B antigen affinity purified polyclonal antibody (A03558) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for CPT1B at approximately 88 kDa. The expected band size for CPT1B is at 88 kDa.

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  IF analysis of CPT1B using anti-CPT1B antibody (A03558).
  CPT1B was detected in an immunocytochemical section of Hela cells. The section was incubated with rabbit anti-CPT1B Antibody (A03558) at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).

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  Flow Cytometry analysis of Hela cells using anti-CPT1B antibody (A03558).
  Overlay histogram showing Hela cells stained with A03558 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-CPT1B Antibody (A03558) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

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  Western blot analysis of CPT1B using anti-CPT1B antibody (A03558). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: monkey heart tissue lysates,
  Lane 2: rat heart tissue lysates,
  Lane 3: mouse heart tissue lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-CPT1B antigen affinity purified polyclonal antibody (A03558) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for CPT1B at approximately 88 kDa. The expected band size for CPT1B is at 88 kDa.

• 

  IF analysis of CPT1B using anti-CPT1B antibody (A03558).
  CPT1B was detected in an immunocytochemical section of Hela cells. The section was incubated with rabbit anti-CPT1B Antibody (A03558) at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).

• 

  Flow Cytometry analysis of Hela cells using anti-CPT1B antibody (A03558).
  Overlay histogram showing Hela cells stained with A03558 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-CPT1B Antibody (A03558) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

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