Anti-CPT1B Antibody

Rabbit polyclonal antibody

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筛选器： All WB IF IHC

PB9491

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of CPT1B using anti-CPT1B antibody (PB9491). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat heart tissue lysates,
Lane 2: rat skeletal tissue lysates,
Lane 3: muscle whole cell lysates,
Lane 4: mouse heart tissue lysates,
Lane 5: mouse skeletal tissue lysates,
Lane 6: muscle whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-CPT1B antigen affinity purified polyclonal antibody (PB9491) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for CPT1B at approximately 88 kDa. The expected band size for CPT1B is at 88 kDa.
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IF analysis using anti- CPT1B Antibody (PB9491). detected in paraffin-embedded section of mouse cardiac tissue. The tissue section were stained using the Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green) (Catalog # BA1127) and counterstained with DAPI (blue).
all(7)
IF analysis using anti- CPT1B Antibody (PB9491). detected in paraffin-embedded section of rat cardiac tissue. The tissue section were stained using the Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green) (Catalog # BA1127) and counterstained with DAPI (blue).
all(7)
IHC analysis of CPT1B using anti-CPT1B antibody (PB9491).
CPT1B was detected in a paraffin-embedded section of mouse skeletal muscle tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-CPT1B Antibody (PB9491) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(7)
IHC analysis of CPT1B using anti-CPT1B antibody (PB9491).
CPT1B was detected in a paraffin-embedded section of Rat Cardiac Muscle tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-CPT1B Antibody (PB9491) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(7)
IHC analysis of CPT1B using anti-CPT1B antibody (PB9491).
CPT1B was detected in a paraffin-embedded section of mouse cardiac muscle tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-CPT1B Antibody (PB9491) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(7)
IHC analysis of CPT1B using anti-CPT1B antibody (PB9491).
CPT1B was detected in a paraffin-embedded section of rat cardiac muscle tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-CPT1B Antibody (PB9491) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(7)

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产品简介>

产品名称

Anti-CPT1B Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

CPT I; CPT1 M; CPT1B; CPT1B-specific; CPTI M; KIAA1670; M CPT1; MCCPT1

产品类型

Polyclonal

检验物种

mouse, rat

应用范围

WB, IF, IHC

基因名称

CPT1B

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence at the N-terminus of human CPT1B, different from the related mouse sequence by two amino acids, and from the related rat sequence by four amino acids.

计算分子量

88 kDa

实际分子量

88 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

CPT1B is located on 22q13.33. The protein encoded by this gene, a member of the carnitine/ choline acetyltransferase family, is the rate-controlling enzyme of the long-chain fatty acid beta-oxidation pathway in muscle mitochondria. This enzyme is required for the net transport of long-chain fatty acyl-CoAs from the cytoplasm into the mitochondria. Multiple transcript variants encoding different isoforms have been found for this gene, and read-through transcripts are expressed from the upstream locus that include exons from this gene.

Uniprot ID

Q92523

RRID

AB_3082139

文献引用格式

CPT1B Antibody (Boster Biological Technology, Wuhan, China. Catalog#PB9491)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

carnitine palmitoyltransferase 1B (muscle)

蛋白名全称

Carnitine O-palmitoyltransferase 1, muscle isoform

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实验步骤

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