Anti-c-Fos/FOS Antibody

Rabbit polyclonal antibody

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筛选器： All WB

BA0207-2

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of c-Fos/FOS using anti-c-Fos/FOS antibody (BA0207-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: HT1080 whole cell lysates,
Lane 2: COLO320 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-c-Fos/FOS antigen affinity purified polyclonal antibody (BA0207-2) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for c-Fos/FOS at approximately 50 kDa. The expected band size for c-Fos/FOS is at 41 kDa.
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产品简介>

产品名称

Anti-c-Fos/FOS Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

AP 1; C FOS; Cellular oncogene fos; c-Fos; FOS; G0S7; Proto oncogene c Fos

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

基因名称

FOS

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence in the middle region of human c-Fos, identical to the related rat and mouse sequences.

计算分子量

41 kDa

实际分子量

50 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

The human oncogene c-fos is cellular homolog of the transforming gene of Finkel-Biskis-Jinkins(FBJ) murine osteosarcoma virus which was mapped to a single human chromosome. c-Fos is encoded by the FOS gene. FOS was the first transcription factor identified that has a critical function in regulating the development of cells destined to form and maintain the skeleton. FOS is also a major component of the activator protein-1(AP-1) transcription factor complex, which includes members of the JUN family. c-fos is a major nuclear target for signal transduction pathways involved in the regulation of cell growth, differentiation, and transformation. Using transgenic and knockout mice, Grigoriadis et al.(1995) established a unique role for the proto-oncogene and nuclear transcription factor, Fos, in regulating the differentiation and activity of specific bone cell populations, both during normal development and in bone disease.

Uniprot ID

P01100

文献引用格式

c-Fos/FOS Antibody (Boster Biological Technology, Wuhan, China. Catalog#BA0207-2)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

Fos proto-oncogene, AP-1 transcription factor subunit

蛋白名全称

Proto-oncogene c-Fos

实验方案>

实验步骤

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