Anti-CCR5 Antibody

Rabbit polyclonal antibody

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筛选器： All WB FCM

BA0376-2

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of CCR5 using anti-CCR5 antibody (BA0376-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: Jurkat whole cell lysates,
Lane 2: Jurkat whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-CCR5 antigen affinity purified polyclonal antibody (BA0376-2) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for CCR5 at approximately 40 kDa. The expected band size for CCR5 is at 41 kDa.
all(2)
Flow cytometry analysis of human PBMC cell(1:100) DyLight 488 conjugated goat anti-rabbit IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).
all(2)

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产品简介>

产品名称

Anti-CCR5 Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

C C chemokine receptor type 5; C C CKR 5; CC CKR 5; CCCKR5; CCR 5; CCR5; CD195; CHEMR13; CKR 5; CKR5; CMKBR5; HIV 1 fusion coreceptor; IDDM22

产品类型

Polyclonal

检验物种

human

应用范围

WB, FCM

基因名称

CCR5

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence in the middle region of human CCR5.

计算分子量

41 kDa

实际分子量

40 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

Human CC chemokine receptor 5(CCR5), which is 17.5 kb from the CMKBR2 gene, encodes a 352-amino acid protein with a calculated molecular mass of 40,600 Da. It is organized into four exons and two introns. Human CCR5 is a co-receptor for macrophage-tropic strains of human immunodeficiency virus(HIV)-1 and is expressed by bone-marrow-derived cells. CCR5 is expressed on neurons, astrocytes, and microglia in the central nervous system, and on epithelium, endothelium, vascular smooth muscle and fibroblasts in other tissues. Functionally, CCR5 mediates the activation of cells by the chemokines macrophage inflammatory protein-1alpha, macrophage inflammatory protein-1beta, and RANTES, and serves as a fusion cofactor for macrophage-tropic strains of human immunodeficiency virus type 1.

Uniprot ID

P51681

文献引用格式

CCR5 Antibody (Boster Biological Technology, Wuhan, China. Catalog#BA0376-2)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

chemokine(C-C motif) receptor 5(gene/pseudogene)

蛋白名全称

C-C chemokine receptor type 5

实验方案>

实验步骤

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