Anti-BCL2 Antibody

Rabbit polyclonal antibody

说明书

筛选器： All IHC WB

BA0412

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

加入购物车立即购买

Figure 1. IHC analysis of BCL2 using anti-BCL2 antibody (BA0412).
BCL2 was detected in a paraffin-embedded section of human rectal cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-BCL2 Antibody (BA0412) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1022) as the chromogen.
all(2)
Figure 2. Western blot analysis of BCL2 using anti-BCL2 antibody (BA0412). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat liver tissue lysates,
Lane 2: rat spleen tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-BCL2 antigen affinity purified polyclonal antibody (BA0412) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for BCL2 at approximately 26 kDa. The expected band size for BCL2 is at 26 kDa.
all(2)

产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-BCL2 Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

Apoptosis regulator Bcl 2; B cell CLL/lymphoma 2; BCL2; BCL-2; Oncogene B cell leukemia 2

产品类型

Polyclonal

检验物种

human, mouse, rabbit, rat

应用范围

IHC, WB

基因名称

BCL2

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence in the middle region of human Bcl-2 identical to the related mouse and rat sequences.

计算分子量

26 kDa

实际分子量

26 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

Immunoreactive BCL2 protein in the neoplastic cells of almost all follicular lymphomas whereas no BCL2 protein was detected in follicles affected by nonneoplastic processes or in normal lymphoid tissue. Every tumor with molecular-genetic evidence of t(14;18) translocation expressed detectable levels of BCL2 protein, regardless of whether the breakpoint was located in or at a distance from the BCL2 gene. Overexpression of BCL2 blocks the apoptotic death of a pro-B-lymphocyte cell line.

Uniprot ID

P10415

文献引用格式

BCL2 Antibody (Boster Biological Technology, Wuhan, China. Catalog#BA0412)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光; ICC－免疫细胞化学; IHC-F- 冰冻切片免疫组化; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

B-cell CLL/lymphoma 2

蛋白名全称

Apoptosis regulator Bcl-2

实验方案>

实验步骤

引用文献(0)>

清除