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Anti-BCL2 Antibody (Clone#Bcl-2-100)

Mouse monoclonal antibody

说明书

筛选器: All WB IHC ICC/IF

BM0200

  • 50μl ¥1280 100μl ¥2180 150μl ¥2800
  • 货期: 现货
  • Lane 1: human 22RV1 whole cell lysates,
    Lane 2: human Hela whole cell lysates,
    Lane 3: human COLO-320 whole cell lysates,
    Lane 4: human PANC-1 whole cell lysates,
    Lane 5: human Jurkat whole cell lysates.

    all(4)
  • IHC(P): Human Intestinal Cancer Tissue

    all(4)
  • IHC(P): Human Mammary Cancer Tissue

    all(4)

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产品名称
Anti-BCL2 Antibody (Clone#Bcl-2-100)
规格/价格
50μl/1280 100μl/2180 150μl/2800
指标别称
Apoptosis regulator Bcl 2; B cell CLL/lymphoma 2; BCL2; BCL-2; Oncogene B cell leukemia 2
产品类型
Monoclonal
检验物种
human
应用范围
WB, IHC, ICC/IF
基因名称
BCL2
克隆号
Bcl-2-100
宿主
Mouse
抗体亚型
IgG1
免疫原
Polypeptide
计算分子量
26 kDa
实际分子量
26 kDa
成分
200ug/ml antibody with PBS ,0.02% NaN3 , 1mg BSA and 50% glycerol.
纯化方式
Ascites
浓度
200ug/ml
产品形态
Liquid
保存条件
12 months from date of receipt,-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.
背景资料
Immunoreactive BCL2 protein in the neoplastic cells of almost all follicular lymphomas whereas no BCL2 protein was detected in follicles affected by nonneoplastic processes or in normal lymphoid tissue. Every tumor with molecular-genetic evidence of t(14;18) translocation expressed detectable levels of BCL2 protein, regardless of whether the breakpoint was located in or at a distance from the BCL2 gene. Overexpression of BCL2 blocks the apoptotic death of a pro-B-lymphocyte cell line.
Uniprot ID
P10415  
文献引用格式
BCL2 Antibody (Clone#Bcl-2-100) (Boster Biological Technology, Wuhan, China. Catalog#BM0200)
应用释义
WB- 蛋白质免疫印迹法,IHC- 免疫组织化学法,ICC/IF-免疫细胞荧光,ICC-免疫细胞化学,IHC-F- 冰冻切片免疫组化,FCM-流式细胞术,ELISA-酶联免疫吸附测定,IP-免疫沉淀法 ,IF-免疫组织荧光法,ChIP-染色质免疫沉淀法
推荐配套的二抗和检测试剂
Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and ICC.
基因名全称
B-cell CLL/lymphoma 2
蛋白名全称
Apoptosis regulator Bcl-2
推荐稀释比
Western blot (WB):1:500-2000
Immunohistochemistry (IHC):1:50-400
Immunocytochemistry/Immunofluorescence (ICC/IF):1:50-400
(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user.

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    Anti-BCL2 Antibody (Clone#Bcl-2-100)

    筛选器: All WB IHC ICC/IF

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