Anti-Beta Catenin/CTNNB1 Antibody

Rabbit polyclonal antibody

说明书

筛选器： All WB IHC ICC/IF

BA0426

50μl ￥1180 100μl ￥1960 150μl ￥2780
货期：现货

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Figure 1. Western blot analysis of Beta Catenin/CTNNB1 using anti-Beta Catenin/CTNNB1 antibody (BA0426). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: rat heart tissue lysates,
Lane 3: rat testis tissue lysates,
Lane 4: human MCF-7 whole cell lysates,
Lane 5: human Hela whole cell lysates,
Lane 6: human M453 whole cell lysates,
Lane 7: human M231 whole cell lysates,
Lane 8: human HT1080 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Beta Catenin/CTNNB1 antigen affinity purified polyclonal antibody (BA0426) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Beta Catenin/CTNNB1 at approximately 95 kDa. The expected band size for Beta Catenin/CTNNB1 is at 85 kDa.
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Figure 2. IHC analysis of Beta Catenin/CTNNB1 using anti-Beta Catenin/CTNNB1 antibody (BA0426) .
Beta Catenin/CTNNB1 was detected in a paraffin-embedded section of human mammary cancer tissue. The tissue section was incubated with rabbit anti-Beta Catenin/CTNNB1 Antibody (BA0426) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
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Figure 3. IF analysis of Beta Catenin/CTNNB1 using anti-Beta Catenin/CTNNB1 antibody (BA0426).
Beta Catenin/CTNNB1 was detected in an immunocytochemical section of A431 cells. The section was incubated with rabbit anti-Beta Catenin/CTNNB1 Antibody (BA0426) at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
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产品简介>

产品名称

Anti-Beta Catenin/CTNNB1 Antibody

规格/价格

50μl/1180 100μl/1960 150μl/2780

指标别称

b cat; Beta catenin; beta-Catenin; Catenin beta 1; CTNNB; CTNNB1; PRO2286; β catenin; β-catenin

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, ICC/IF

基因名称

CTNNB1

抗体来源

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence at the N-terminus of human beta Catenin, identical to the related rat and mouse sequences.

计算分子量

85 kDa

实际分子量

95 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

Catenins are proteins found in complexes with cadherin cell adhesion molecules of animal cells. The first two catenins that were identified became known as alpha-catenin and beta-catenin. Alpha-catenin can bind to beta-catenin and can also bind actin. Beta-catenin binds the cytoplasmic domain of some cadherins. Beta-catenin is an adherens junction protein. It plays an important role in various aspects of liver biology including liver development(both embryonic and postnatal), liver regeneration following partial hepatectomy. HGF-induced hepatpomegaly, liver zonation, and pathogenesis of liver cancer.

Uniprot ID

P35222

文献引用格式

Beta Catenin/CTNNB1 Antibody (Boster Biological Technology, Wuhan, China. Catalog#BA0426)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光; ICC－免疫细胞化学; IHC-F- 冰冻切片免疫组化; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

catenin(cadherin-associated protein), beta 1, 88kDa

蛋白名全称

Catenin beta-1

实验方案>

实验步骤

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