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Anti-Beta Catenin/CTNNB1 Antibody (Clone#OTI1F3)

Mouse monoclonal antibody

说明书

筛选器: All WB IHC

M00004-6

  • 50μl ¥1180 100μl ¥1960 150μl ¥2780
  • 货期: 5-7天
  • Figure 1. Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CTNNB1 monoclonal antibody.

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  • Figure 2. Immunohistochemical staining of paraffin-embedded Human lung tissue within the normal limits using anti-CTNNB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M00004-6)

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  • Figure 3. HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CTNNB1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CTNNB1.

    all(3)

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产品简介>

产品名称
Anti-Beta Catenin/CTNNB1 Antibody (Clone#OTI1F3)
规格/价格
50μl/1180 100μl/1960 150μl/2780
指标别称
b cat; Beta catenin; beta-Catenin; Catenin beta 1; CTNNB; CTNNB1; PRO2286; β catenin; β-catenin
产品类型
Monoclonal
检验物种
human, monkey, mouse, rat, dog
应用范围
WB, IHC
基因名称
CTNNB1
克隆号
OTI1F3
抗体来源
Mouse
抗体亚型
IgG2a
免疫原
Human recombinant protein fragment corresponding to amino acids 531-781 of human beta-catenin (NP_001895) produced in E.coli.
计算分子量
85 kDa
实际分子量
85.3 kDa
成分
PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
纯化方式
Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
浓度
500 ug/ml
产品形态
Liquid
保存条件
Stable for 12 months from date of receipt. Store at -20°C as received.
背景资料
Catenins are proteins found in complexes with cadherin cell adhesion molecules of animal cells. The first two catenins that were identified became known as alpha-catenin and beta-catenin. Alpha-catenin can bind to beta-catenin and can also bind actin. Beta-catenin binds the cytoplasmic domain of some cadherins. Beta-catenin is an adherens junction protein. It plays an important role in various aspects of liver biology including liver development(both embryonic and postnatal), liver regeneration following partial hepatectomy. HGF-induced hepatpomegaly, liver zonation, and pathogenesis of liver cancer.
Uniprot ID
P35222  
文献引用格式
Beta Catenin/CTNNB1 Antibody (Clone#OTI1F3) (Boster Biological Technology, Wuhan, China. Catalog#M00004-6)
应用释义
WB- 蛋白质免疫印迹法,IHC- 免疫组织化学法,ICC/IF-免疫细胞荧光,ICC-免疫细胞化学,IHC-F- 冰冻切片免疫组化,FCM-流式细胞术,ELISA-酶联免疫吸附测定,IP-免疫沉淀法 ,IF-免疫组织荧光法,ChIP-染色质免疫沉淀法
基因名全称
Catenin beta-1
蛋白名全称
Catenin beta-1
推荐稀释比
Western blot (WB):1:1000~2000
Immunohistochemistry (IHC):1:50

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    Anti-Beta Catenin/CTNNB1 Antibody (Clone#OTI1F3)

    筛选器: All WB IHC

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