Anti-Calpain 1/CAPN1 Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC ICC/IF

BA0679

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of Calpain 1/CAPN1 using anti-Calpain 1/CAPN1 antibody (BA0679). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human Jurkat whole cell lysates,
Lane 3: human RT4 whole cell lysates,
Lane 4: human A431 whole cell lysates,
Lane 5: rat lung tissue lysates,
Lane 6: mouse lung tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Calpain 1/CAPN1 antigen affinity purified polyclonal antibody (BA0679) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Calpain 1/CAPN1 at approximately 82 kDa. The expected band size for Calpain 1/CAPN1 is at 82 kDa.
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IHC analysis of Calpain 1/CAPN1 using anti-Calpain 1/CAPN1 antibody (BA0679).
Calpain 1/CAPN1 was detected in a paraffin-embedded section of rat lung tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Calpain 1/CAPN1 Antibody (BA0679) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
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IHC analysis of Calpain 1/CAPN1 using anti-Calpain 1/CAPN1 antibody (BA0679).
Calpain 1/CAPN1 was detected in a paraffin-embedded section of human intestinal cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Calpain 1/CAPN1 Antibody (BA0679) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(6)
IHC analysis of Calpain 1/CAPN1 using anti-Calpain 1/CAPN1 antibody (BA0679).
Calpain 1/CAPN1 was detected in frozen section of rat cardiac muscle tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Calpain 1/CAPN1 Antibody (BA0679) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(6)
ICC analysis of Calpain 1/CAPN1 using anti- Calpain 1/CAPN1 antibody (BA0679).
Calpain 1/CAPN1 was detected in an immunocytochemical section of Hela cells. The section was incubated with rabbit anti-Calpain 1/CAPN1 Antibody (BA0679) at a dilution of 1:100. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(6)
IF analysis of Calpain 1/CAPN1 using anti-Calpain 1/CAPN1 antibody (BA0679).
Calpain 1/CAPN1 was detected in an immunocytochemical section of A549 cells. The section was incubated with rabbit anti-Calpain 1/CAPN1 Antibody (BA0679) at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
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产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-Calpain 1/CAPN1 Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

Calpain 1; Calpain 1 catalytic subunit; Calpain 1 large subunit; Calpain mu type; CANP; CANP 1; CANP1; CANPL1; CAPN1; Micromolar calpain; muCANP; muCL

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, ICC/IF

基因名称

CAPN1

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence in the middle region of human Calpain 1, different from the mouse sequence by two amino acids.

计算分子量

82 kDa

实际分子量

82 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

Calpain-1 catalytic subunit is a protein that in humans is encoded by the CAPN1 gene. Calpain is an intracellular protease that requires calcium for its catalytic activity. Two isozymes, calpain I(mu-calpain) and calpain II(m-calpain), with different calcium requirements, have been identified. Both are heterodimers composed of L(large, catalytic, 80 kD) and S(small, regulatory, 30 kD) subunits. The isozymes share an identical S subunit, with the differences arising from the L subunits, L1(CAPN1) and L2. By quantitative RT-PCR, Ueyama et al.(1998) found that expression of calpain-1 and calpain-2 mRNA was significantly increased in muscle biopsy samples derived from 5 men with progressive muscular dystrophy(e.g., DMD; 310200) and 2 men and 3 women with amyotrophic lateral sclerosis(ALS; 105400) compared with controls. Using cDNA clones as probes, Ohno et al.(1989, 1990) assign CANPL1 to chromosome 11.

Uniprot ID

P07384

文献引用格式

Calpain 1/CAPN1 Antibody (Boster Biological Technology, Wuhan, China. Catalog#BA0679)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

calpain 1,(mu/I) large subunit

蛋白名全称

Calpain-1 catalytic subunit

实验方案>

实验步骤

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