Anti-Calpain 1/CAPN1 Antibody (Clone#2E3)

筛选器： All FCM WB

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  Flow Cytometry analysis of A549 cells using anti-Calpain 1/CAPN1 antibody (M01943-4).
  Overlay histogram showing A549 cells stained with M01943-4 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with mouse anti-Calpain 1/CAPN1 Antibody (M01943-4) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

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  Western blot analysis of Calpain 1/CAPN1 using anti-Calpain 1/CAPN1 antibody (M01943-4). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human Jurkat whole cell lysates,
  Lane 2: human Hela whole cell lysates,
  Lane 3: human K562 whole cell lysates,
  Lane 4: human PC-3 whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-Calpain 1/CAPN1 antigen affinity purified monoclonal antibody (M01943-4) at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Calpain 1/CAPN1 at approximately 82 kDa. The expected band size for Calpain 1/CAPN1 is at 82 kDa.

• 

  Flow Cytometry analysis of A549 cells using anti-Calpain 1/CAPN1 antibody (M01943-4).
  Overlay histogram showing A549 cells stained with M01943-4 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with mouse anti-Calpain 1/CAPN1 Antibody (M01943-4) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

• 

  Western blot analysis of Calpain 1/CAPN1 using anti-Calpain 1/CAPN1 antibody (M01943-4). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human Jurkat whole cell lysates,
  Lane 2: human Hela whole cell lysates,
  Lane 3: human K562 whole cell lysates,
  Lane 4: human PC-3 whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-Calpain 1/CAPN1 antigen affinity purified monoclonal antibody (M01943-4) at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Calpain 1/CAPN1 at approximately 82 kDa. The expected band size for Calpain 1/CAPN1 is at 82 kDa.