Anti-Cyclin B1/CCNB1 Antibody

Rabbit polyclonal antibody

说明书

筛选器： All WB

BA0766-2

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Figure 1. Western blot analysis of Cyclin B1/CCNB1 using anti-Cyclin B1/CCNB1 antibody (BA0766-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: rat thymus tissue lysates,
Lane 3: rat placenta tissue lysates,
Lane 4: rat spleen tissue lysates,
Lane 5: human Raji whole cell lysates,
Lane 6: human MCF-7 whole cell lysates,
Lane 7: human COLO320 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Cyclin B1/CCNB1 antigen affinity purified polyclonal antibody (BA0766-2) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Cyclin B1/CCNB1 at approximately 55 kDa. The expected band size for Cyclin B1/CCNB1 is at 48 kDa.
all(2)
Figure 2. Western blot analysis of Cyclin B1/CCNB1 using anti-Cyclin B1/CCNB1 antibody (BA0766-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat testis tissue lysates,
Lane 2: rat ovary tissue lysates,
Lane 3: rat thymus tissue lysates,
Lane 4: rat spleen tissue lysates,
Lane 5: human Hela whole cell lysates,
Lane 6: human 293T whole cell lysates,
Lane 7: human SW620 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Cyclin B1/CCNB1 antigen affinity purified polyclonal antibody (BA0766-2) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Cyclin B1/CCNB1 at approximately 55 kDa. The expected band size for Cyclin B1/CCNB1 is at 48 kDa.
all(2)

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产品简介>

产品名称

Anti-Cyclin B1/CCNB1 Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

CCNB; CCNB1; cyclin B1; G2/mitotic specific cyclin B1

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

基因名称

CCNB1

宿主

Rabbit

抗体亚型

IgG

免疫原

E.coli-derived human Cyclin B1 recombinant protein (Position: M1-V433). Human Cyclin B1 shares 86% and 85% amino acid (aa) sequences identity with mouse and rat Cyclin B1, respectively.

计算分子量

48 kDa

实际分子量

55 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

CCNB also known as Cyclin B1, is a protein that in humans is encoded by the CCNB1 gene, and it is mapped to 5q13.2. The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. CCNB contributes to the switch-like all or none behavior of the cell in deciding to commit to mitosis. Its activation is well-regulated, and positive feedback loops ensure that once the cyclin B1-Cdk1 complex is activated, it is not deactivated.

Uniprot ID

P14635

文献引用格式

Cyclin B1/CCNB1 Antibody (Boster Biological Technology, Wuhan, China. Catalog#BA0766-2)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光; ICC－免疫细胞化学; IHC-F- 冰冻切片免疫组化; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

cyclin B1

蛋白名全称

G2/mitotic-specific cyclin-B1

实验方案>

实验步骤

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