| Western blot (WB): | 1:500-2000 |

Western blot analysis of FOSB using anti-FOSB antibody (BA0866-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: HT1080 whole cell lysates ,
Lane 2: SW620 whole cell lysates ,
Lane 3: HELA whole cell lysates ,
Lane 4: SMMC whole cell lysates ,
Lane 5: MM453 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-FOSB antigen affinity purified polyclonal antibody (BA0866-2) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for FOSB at approximately 36 kDa. The expected band size for FOSB is at 36 kDa.

Western blot analysis of FOSB using anti-FOSB antibody (BA0866-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: HT1080 whole cell lysates ,
Lane 2: SW620 whole cell lysates ,
Lane 3: HELA whole cell lysates ,
Lane 4: SMMC whole cell lysates ,
Lane 5: MM453 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-FOSB antigen affinity purified polyclonal antibody (BA0866-2) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for FOSB at approximately 36 kDa. The expected band size for FOSB is at 36 kDa.
