Anti-FOSB Antibody (Clone#DFO-6)

Rabbit monoclonal antibody

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筛选器： All WB IHC ICC/IF

BM4319

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of anti-FOSB antibody (BM4319). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human RT4 whole cell lysates,
Lane 2: human U2OS whole cell lysates,
Lane 3: human SiHa whole cell lysates,
Lane 4: rat brain tissue lysates,
Lane 5: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-FOSB antigen affinity purified monoclonal antibody (BM4319) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for FOSB at approximately 45 kDa. The expected band size for FOSB is at 36 kDa.
all(4)
IHC analysis of FOSB using anti-FOSB antibody (BM4319) .
FOSB was detected in a paraffin-embedded section of mouse brain tissue. The tissue section was incubated with rabbit anti-FOSB Antibody (BM4319) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(4)
IHC analysis of FOSB using anti-FOSB antibody (BM4319) .
FOSB was detected in a paraffin-embedded section of rat brain tissue. The tissue section was incubated with rabbit anti-FOSB Antibody (BM4319) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(4)
IF analysis of FOSB using anti-FOSB antibody (BM4319) and anti-Beta Tubulin antibody (M02533-3).
FOSB was detected in an immunocytochemical section of Hela cells. Cy3-conjugated Anti-mouse IgG Secondary Antibody (red)(Catalog#BA1031) and Dylight488-conjugated Anti-mouse IgG Secondary Antibody (green)(Catalog#BA1126) was used as secondary antibody.
all(4)

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产品简介>

产品名称

Anti-FOSB Antibody (Clone#DFO-6)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

FosB proto-oncogene; AP-1 transcription factor subunit; AP-1; G0S3; GOS3; GOSB; FOSB

产品类型

Monoclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, ICC/IF

基因名称

FOSB

克隆号

DFO-6

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthesized peptide derived from human Fos B

计算分子量

36 kDa

实际分子量

36 kDa

成分

500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.

纯化方式

Affinity-chromatography

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

FOSB, FBJ murine osteosarcoma viral oncogene homolog B, is a protein that, in humans, is encoded by the FOSB gene. FOSB is a member of Fos gene family which consists of 4 members: FOS, FOSB, FOSL1, and FOSL2. The FOS proteins have been implicated as regulators of cell proliferation, differentiation, and transformation. The FOSB gene is mapped to 19q13.32. Delta FOSB is a truncated splice variant of FOSB. Delta FosB has been implicated in the development of drug addiction and control of the reward system in the brain, and is linked to changes in a number of other gene products such as CREB and sirtuins. Delta FosB also regulates the commitment of mesenchymal precursor cells to the adipocyte or osteoblast lineage.

Uniprot ID

P53539

文献引用格式

FOSB Antibody (Clone#DFO-6) (Boster Biological Technology, Wuhan, China. Catalog#BM4319)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

Protein fosB

实验方案>

实验步骤

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