Anti-MT1-MMP/MMP14 Antibody

Rabbit polyclonal antibody

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筛选器： All IHC WB

BA1278

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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IHC analysis of MT1-MMP/MMP14 using anti-MT1-MMP/MMP14 antibody (BA1278).
MT1-MMP/MMP14 was detected in a paraffin-embedded section of human intestinal cancer tissue. The tissue section was incubated with rabbit anti-MT1-MMP/MMP14 Antibody (BA1278) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
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IHC analysis of MT1-MMP/MMP14 using anti-MT1-MMP/MMP14 antibody (BA1278).
MT1-MMP/MMP14 was detected in a paraffin-embedded section of human placenta tissue. The tissue section was incubated with rabbit anti-MT1-MMP/MMP14 Antibody (BA1278) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(4)
IHC analysis of MT1-MMP/MMP14 using anti-MT1-MMP/MMP14 antibody (BA1278).
MT1-MMP/MMP14 was detected in a paraffin-embedded section of rat cardiac muscle tissue. The tissue section was incubated with rabbit anti-MT1-MMP/MMP14 Antibody (BA1278) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(4)
Western blot analysis of MT1-MMP/MMP14 using anti-MT1-MMP/MMP14 antibody (BA1278). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: Rat Spleen tissue lysates at 50ug,
Lane 2: Human Placenta tissue lysates at 50ug,
Lane 3: HELA whole cell lysates at 40ug,
Lane 4: A549 whole cell lysates at 40ug.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-MT1-MMP/MMP14 antigen affinity purified polyclonal antibody (BA1278) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for MT1-MMP/MMP14 at approximately 60-65 kDa. The expected band size for MT1-MMP/MMP14 is at 66 kDa.
all(4)

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产品简介>

产品名称

Anti-MT1-MMP/MMP14 Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

Matrix metalloproteinase 14; MMP 14; MMP X1; MMP14; MT MMP 1; MT1 MMP; MT1MMP; MT1-MMP; MTMMP1

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

IHC, WB

基因名称

MMP14

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence at the C-terminus of human MMP14, different from the related mouse and rat sequences by one amino acid.

计算分子量

66 kDa

实际分子量

60-65 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

Matrix metalloproteinase-14, also called MT-MMP 1, is an enzyme that in humans is encoded by the MMP14 gene. The protein encoded by this gene is a member of the membrane-type MMP subfamily. This gene is mapped to 14q11.2. Proteins of the matrix metalloproteinase(MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. This gene may be involved in actin cytoskeleton reorganization by cleaving PTK7. It acts as a positive regulator of cell growth and migration via activation of MMP15.

Uniprot ID

P50281

文献引用格式

MT1-MMP/MMP14 Antibody (Boster Biological Technology, Wuhan, China. Catalog#BA1278)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

matrix metallopeptidase 14(membrane-inserted)

蛋白名全称

Matrix metalloproteinase-14

实验方案>

实验步骤

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