Anti-MIF Antibody

Rabbit polyclonal antibody

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筛选器： All WB ELISA

BA14437

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of MIF using anti-MIF antibody (BA14437). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Jurkat whole cell lysates,
Lane 2: human K562 whole cell lysates,
Lane 3: human 293T whole cell lysates,
Lane 4: human PC-3 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-MIF antigen affinity purified polyclonal antibody (BA14437) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for MIF at approximately 12 kDa. The expected band size for MIF is at 12 kDa.
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产品简介>

产品名称

Anti-MIF Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

GIF; GLIF; L dopachrome isomerase; L dopachrome tautomerase; MIF; MMIF; Phenylpyruvate tautomerase

产品类型

Polyclonal

检验物种

human

应用范围

WB, ELISA

基因名称

MIF

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence at the C-terminus of human MIF, different from the related rat and mouse sequences by one amino acid.

计算分子量

12 kDa

实际分子量

12 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

Macrophage migration inhibitory factor, MIF, is a cytokine released by T-lymphocytes, macrophages, and the pituitary gland that serves to integrate peripheral and central inflammatory responses. MIF gene has 3 exons separated by introns of only 189 and 95 bp, and covers less than 1 kb. Localization of the human gene for macrophage migration inhibitory factor(MIF) to chromosome 22q11.2 MIF plays a critical role in inflammatory diseases and atherogenesis.

Uniprot ID

P14174

文献引用格式

MIF Antibody (Boster Biological Technology, Wuhan, China. Catalog#BA14437)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

macrophage migration inhibitory factor(glycosylation-inhibiting factor)

蛋白名全称

Macrophage migration inhibitory factor(MIF)

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实验步骤

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