Western blot analysis of MIF using anti-MIF antibody (BA2058). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Jurkat whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: rat kidney tissue lysates,
Lane 4: rat NRK whole cell lysates,
Lane 5: rat PC-12 whole cell lysates,
Lane 6: mouse kidney tissue lysates,
Lane 7: mouse HBZY-1 whole cell lysates,
Lane 8: mouse RAW264.7 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-MIF antigen affinity purified polyclonal antibody (BA2058) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for MIF at approximately 12 kDa. The expected band size for MIF is at 12 kDa.
IHC analysis of MIF using anti-MIF antibody (BA2058) .
MIF was detected in a paraffin-embedded section of mouse brain tissue. The tissue section was incubated with rabbit anti-MIF Antibody (BA2058) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of MIF using anti-MIF antibody (BA2058) .
MIF was detected in a paraffin-embedded section of rat brain tissue. The tissue section was incubated with rabbit anti-MIF Antibody (BA2058) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
Flow Cytometry analysis of K562 cells using anti-MIF antibody (BA2058).
Overlay histogram showing K562 cells stained with BA2058 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MIF Antibody (BA2058, 1:100). DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 1:100) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG (Catalog # BA1045) (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
| Western blot (WB): | 1:500-2000 |
| Immunohistochemistry (IHC): | 1:50-400 |
| Flow Cytometry (Fixed): | 1:50-200 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
Western blot analysis of MIF using anti-MIF antibody (BA2058). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Jurkat whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: rat kidney tissue lysates,
Lane 4: rat NRK whole cell lysates,
Lane 5: rat PC-12 whole cell lysates,
Lane 6: mouse kidney tissue lysates,
Lane 7: mouse HBZY-1 whole cell lysates,
Lane 8: mouse RAW264.7 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-MIF antigen affinity purified polyclonal antibody (BA2058) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for MIF at approximately 12 kDa. The expected band size for MIF is at 12 kDa.
IHC analysis of MIF using anti-MIF antibody (BA2058) .
MIF was detected in a paraffin-embedded section of mouse brain tissue. The tissue section was incubated with rabbit anti-MIF Antibody (BA2058) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of MIF using anti-MIF antibody (BA2058) .
MIF was detected in a paraffin-embedded section of rat brain tissue. The tissue section was incubated with rabbit anti-MIF Antibody (BA2058) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
Flow Cytometry analysis of K562 cells using anti-MIF antibody (BA2058).
Overlay histogram showing K562 cells stained with BA2058 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MIF Antibody (BA2058, 1:100). DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 1:100) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG (Catalog # BA1045) (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Western blot analysis of MIF using anti-MIF antibody (BA2058). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Jurkat whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: rat kidney tissue lysates,
Lane 4: rat NRK whole cell lysates,
Lane 5: rat PC-12 whole cell lysates,
Lane 6: mouse kidney tissue lysates,
Lane 7: mouse HBZY-1 whole cell lysates,
Lane 8: mouse RAW264.7 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-MIF antigen affinity purified polyclonal antibody (BA2058) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for MIF at approximately 12 kDa. The expected band size for MIF is at 12 kDa.
IHC analysis of MIF using anti-MIF antibody (BA2058) .
MIF was detected in a paraffin-embedded section of mouse brain tissue. The tissue section was incubated with rabbit anti-MIF Antibody (BA2058) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of MIF using anti-MIF antibody (BA2058) .
MIF was detected in a paraffin-embedded section of rat brain tissue. The tissue section was incubated with rabbit anti-MIF Antibody (BA2058) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
Flow Cytometry analysis of K562 cells using anti-MIF antibody (BA2058).
Overlay histogram showing K562 cells stained with BA2058 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MIF Antibody (BA2058, 1:100). DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 1:100) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG (Catalog # BA1045) (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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