Anti-GCLC Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC

BA1627

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of GCLC using anti-GCLC antibody (BA1627). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: Rat Brain tissue lysates,
Lane 2: Rat Kidney tissue lysates,
Lane 3: Rat Heart tissue lysates,
Lane 4: Rat Lung tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-GCLC antigen affinity purified polyclonal antibody (BA1627) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for GCLC at approximately 73 kDa. The expected band size for GCLC is at 73 kDa.
all(2)
IHC analysis of GCLC using anti-GCLC antibody (BA1627).
GCLC was detected in a paraffin-embedded section of human rectal cancer tissue. The tissue section was incubated with rabbit anti-GCLC Antibody (BA1627) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(2)

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产品简介>

产品名称

Anti-GCLC Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

Gamma ECS; GCLC; GCS; GCS heavy chain; GLCL; GLCLC

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

WB, IHC

基因名称

GCLC

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence at the N-terminus of human GCLC, different from the related rat and mouse sequences by one amino acid.

计算分子量

73 kDa

实际分子量

73 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

GCLC, Glutamate--cysteine ligase catalytic subunit, is an enzyme that in humans is encoded by the GCLC gene. Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. The gene encoding the catalytic subunit encodes a protein of 367 amino acids with a calculated molecular weight of 72.773 kDa and maps to chromosome 6p12.1. Deficiency of gamma-glutamylcysteine synthetase in human is associated with enzymopathic hemolytic anemia.

Uniprot ID

P48506

文献引用格式

GCLC Antibody (Boster Biological Technology, Wuhan, China. Catalog#BA1627)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

glutamate-cysteine ligase, catalytic subunit

蛋白名全称

Glutamate--cysteine ligase catalytic subunit

实验方案>

实验步骤

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