Anti-GCLC Antibody

筛选器： All WB IHC FCM

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  Western blot analysis of GCLC using anti-GCLC antibody (PB9201). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human A549 whole cell lysates,
  Lane 2: human RT4 whole cell lysates,
  Lane 3: human HepG2 whole cell lysates,
  Lane 4: human A431 whole cell lysates,
  Lane 5: rat liver tissue lysates,
  Lane 6: rat kidney tissue lysates,
  Lane 7: mouse liver tissue lysates,
  Lane 8: mouse kidney tissue lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-GCLC antigen affinity purified polyclonal antibody (PB9201) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for GCLC at approximately 73 kDa. The expected band size for GCLC is at 73 kDa.

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  IHC analysis of GCLC using anti-GCLC antibody (PB9201) .
  GCLC was detected in a paraffin-embedded section of human colorectal cancer tissue. The tissue section was incubated with rabbit anti-GCLC Antibody (PB9201) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

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  Flow Cytometry analysis of A549 cells using anti-GCLC antibody (PB9201).
  Overlay histogram showing A549 cells stained with PB9201 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GCLC Antibody (PB9201) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample (Red line) was also used as a control.

• 

  Western blot analysis of GCLC using anti-GCLC antibody (PB9201). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human A549 whole cell lysates,
  Lane 2: human RT4 whole cell lysates,
  Lane 3: human HepG2 whole cell lysates,
  Lane 4: human A431 whole cell lysates,
  Lane 5: rat liver tissue lysates,
  Lane 6: rat kidney tissue lysates,
  Lane 7: mouse liver tissue lysates,
  Lane 8: mouse kidney tissue lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-GCLC antigen affinity purified polyclonal antibody (PB9201) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for GCLC at approximately 73 kDa. The expected band size for GCLC is at 73 kDa.

• 

  IHC analysis of GCLC using anti-GCLC antibody (PB9201) .
  GCLC was detected in a paraffin-embedded section of human colorectal cancer tissue. The tissue section was incubated with rabbit anti-GCLC Antibody (PB9201) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

• 

  Flow Cytometry analysis of A549 cells using anti-GCLC antibody (PB9201).
  Overlay histogram showing A549 cells stained with PB9201 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GCLC Antibody (PB9201) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample (Red line) was also used as a control.